Denisova L Ia, Zagrebel'nyĭ S N, Pustoshilova N M, Putintseva N I, Puchkova L I
Mol Biol (Mosk). 1984 May-Jun;18(3):599-606.
A recombinant DNA was constructed by inserting polynucleotide (dA).(dT) of 80-100 base pairs long into EcoRI site of the pBR325 plasmid DNA. Transcription of this DNA was studied in E. coli RNA polymerase system in vitro. Some transcripts obtained with the recombinant plasmid were shown to have poly(U) clusters at the 3'-ends. Obtained data indicate that poly(dA).poly(dT) sequence acts as a terminator of RNA synthesis. Orientation of this sequence in recombinant DNA was also established.
通过将80 - 100个碱基对长的多核苷酸(dA).(dT)插入pBR325质粒DNA的EcoRI位点构建了重组DNA。在体外大肠杆菌RNA聚合酶系统中研究了该DNA的转录。用重组质粒获得的一些转录本在3'端显示有多聚(U)簇。获得的数据表明多聚(dA).多聚(dT)序列作为RNA合成的终止子。还确定了该序列在重组DNA中的方向。
