Helpap B, Grouls V, Yamashita K
Virchows Arch B Cell Pathol. 1975 Nov 21;19(3):269-79. doi: 10.1007/BF02889373.
In order to estimate the role of a possible immunological coeffect on the splenic cellular proliferation during wound healing after mechanical or thermal lesions of internal organs, light microscopical and autoradiographical investigations with tritiated thymidine were performed on spleens of non-germfree rats, immunized by a single injection of sheep erythrocytes. Two days after an initial dissociation of preexisting germinal centers in the spleen a steep rise of the percentages of labeled cells in newly formed germinal centers occurs with a maximum on the fifth day. In the marginal zone an increased cell proliferation (mostly large lymphoid blasts) starts at 12 hours. In the lymphatic mantle zone a marked increase of labeled basophilic blasts (immunoblasts) can be observed with a maximum on the second day. The values are higher in the perifollicular B cell region than in the periarteriolar T cell region. In the red pulp of the spleen the highest percentages of labeled cells occur five days after the antigen injection. These findings in the spleen after a strong antigenic stimulation are characteristic for an immunological reaction of anamnestic type. In comparison with investigations of the spleen after cryolesions in internal organs such studies may be particularly helpful in judging the proportion of an unknown immunological reaction in the spleen after such an operation, which by tissue necroses and cellular destruction may have caused denaturation of self components.
为了评估在内部器官受到机械性或热性损伤后伤口愈合过程中，可能的免疫协同效应在脾细胞增殖中所起的作用，我们对经单次注射绵羊红细胞免疫的非无菌大鼠的脾脏进行了光镜和用氚标记胸腺嘧啶核苷的放射自显影研究。在脾脏中预先存在的生发中心最初解离两天后，新形成的生发中心中标记细胞的百分比急剧上升，并在第五天达到最大值。在边缘区，细胞增殖增加（主要是大淋巴细胞母细胞）在12小时开始。在淋巴套区，可观察到标记的嗜碱性母细胞（免疫母细胞）显著增加，在第二天达到最大值。在滤泡周围B细胞区域的值高于动脉周围T细胞区域。在脾脏红髓中，标记细胞的最高百分比在抗原注射后五天出现。在受到强烈抗原刺激后脾脏中的这些发现是回忆型免疫反应的特征。与对内部器官冷冻损伤后脾脏的研究相比，此类研究可能对判断此类手术后脾脏中未知免疫反应的比例特别有帮助，因为手术可能因组织坏死和细胞破坏而导致自身成分变性。
