Palva T, Palva A
Ann Otol Rhinol Laryngol. 1975 Jan-Feb;84(1 Pt 1):112-6. doi: 10.1177/000348947508400117.
Mucosal biopsies were taken from 20 ears with secretory otitis media (glue ear) and histochemical stainings were made for comparison with data obtained from biochemical analysis of the fluids. Acid phosphatase, lactate dehydrogenase (LD), and malate dehydrogenase (MD), the activity of which in the ear fluids was 20 to 30 times higher than in serum, were found to appear as strong precipitates in the middle ear epithelium, particularly in the top layer. Alkaline phosphatase activity was only exceptionally seen in the epithelium but appeared in the capillaries and histiocytes. Nonspecific esterase appeared irregularly in the epithelium and regularly in histiocytes. The latter two had lower activities in ear fluids than in serum. Epithelial secretory cells and subepithelial glands and cysts showed strong alcian blue (AB)-positive staining. Positive material appeared also in the cytoplasm of the epithelial cells and in the intercellular substance. Distinct PAS-positive staining appeared in the columnar epithelium and particularly in the free mucus on top of the epithelium but was less promounced in the glandular structures and absent from the cysts.
对20例分泌性中耳炎（胶耳）患者的耳部进行黏膜活检，并进行组织化学染色，以便与从耳液生化分析中获得的数据进行比较。酸性磷酸酶、乳酸脱氢酶（LD）和苹果酸脱氢酶（MD）在耳液中的活性比血清中高20至30倍，发现它们在中耳上皮，尤其是顶层上皮中呈现强烈沉淀。碱性磷酸酶活性仅在少数上皮细胞中可见，但在毛细血管和组织细胞中出现。非特异性酯酶在上皮中不规则出现，在组织细胞中规律出现。后两者在耳液中的活性低于血清。上皮分泌细胞、上皮下腺和囊肿显示出强烈的阿尔辛蓝（AB）阳性染色。阳性物质也出现在上皮细胞的细胞质和细胞间质中。柱状上皮，尤其是上皮顶部的游离黏液中出现明显的PAS阳性染色，但在腺结构中不太明显，囊肿中则无。
