Jurin M, Drewinko B
Am J Pathol. 1974 Nov;77(2):213-36.
The morphologic changes in lymphoreticular tissues and development of antitumor immune reactions of specific pathogen-free mice injected with syngeneic lymphoma cells were sequentially analyzed. The regional (right inguinal) lymph node demonstrated mild changes indicative of immunologic response. Systemic lymph nodes revealed a moderate degree of immune response on morphologic basis. The spleen was the site of marked activity, characterized by the presence of large pyroninophilic cells and germinal centers. Foci of necrosis in the local tumor accompanied by mature lymphocytes suggested cell-mediated immune rejection. Mice developed circulating antibodies 2 days after implantation. No antibodies were demonstrated attached to fresh tumor cells. Lymphocyte cytotoxic activity was demonstrated beginning on day 4. Both cytotoxic activity and circulating antibodies were no longer detectable after the third week following tumor implantation. Tumor-bearing mice also had an impaired capacity to mount a primary immune reaction to sheep red blood cells. The spleen demonstrated a marked loss of lymphocytes and the subsequent appearance of masses of amyloid material. It is suggested that amyloidosis in lymphoreticular organs is the result of a derangement in the immune response of the host following a prolonged and sustained antigenic stimulation. It appears that in syngeneic pathogen-free mice the spleen plays the major role in immune rejection mechanisms while the draining node only plays a modest role.
对注射了同基因淋巴瘤细胞的无特定病原体小鼠的淋巴网状组织形态学变化及抗肿瘤免疫反应的发展进行了连续分析。局部（右腹股沟）淋巴结表现出轻度变化，提示有免疫反应。全身淋巴结在形态学基础上显示出中度免疫反应。脾脏是明显活动的部位，其特征是存在大量嗜派洛宁细胞和生发中心。局部肿瘤中的坏死灶伴有成熟淋巴细胞，提示细胞介导的免疫排斥。小鼠在植入后2天产生循环抗体。未发现新鲜肿瘤细胞上附着有抗体。从第4天开始显示淋巴细胞细胞毒性活性。肿瘤植入后第三周后，细胞毒性活性和循环抗体均不再可检测到。荷瘤小鼠对绵羊红细胞产生初次免疫反应的能力也受损。脾脏显示淋巴细胞明显减少，随后出现大量淀粉样物质。提示淋巴网状器官中的淀粉样变性是宿主在长期持续抗原刺激后免疫反应紊乱的结果。看来在同基因无特定病原体小鼠中，脾脏在免疫排斥机制中起主要作用，而引流淋巴结仅起适度作用。
