Milas L, Basic I, Kogelnik H D, Withers H R
Cancer Res. 1975 Sep;35(9):2365-74.
We studied the effect of single and multiple injections of Corynebacterium granulosum on weight and histology of lymph nodes and spleen, on peripheral white blood cell count, response of peripheral blood lymphocytes, lymph node, and spleen cells to phytohemagglutinin and pokeweed mitogen, survival of skin allografts, and lung metastases of a syngeneic fibrosarcoma in C3Hf/Bu mice. Corynebacterium parvum was used in some studies on antitumor activity. The weight of lymph nodes and spleen was markedly increased by single and multiple i.p. injections of C. granulosum, the peak enlargement occurring at Day 7 in lymph nodes and at Day 16 in spleen. Histologically, there was an extensive proliferation of nucleated cells in the enlarged organs. C. granulosum did not change the total white blood cell count but caused a temporary lymphopenia. In general, in vitro response to phytohemagglutinin and pokeweed mitogen of blood lymphocytes and spleen cells was decreased. Lymph node cell response to phytohemagglutinin was increased by small doses (0.025 mg) of C. granulosum, was not altered by a single large dose (0.5 mg), and was decreased by multiple doses. The response of lymph node cells to pokeweed mitogen was increased by all treatments. These changes in response to mitogens were demonstrable for about 2 months after treatment. Treatment i.v. with 0.1 or 0.25 mg of C. granulosum given before but not after grafting significantly prolonged the survival of grafted BALB/c skin. Smaller doses of this bacterium were not effective. Splenectomy of skin graft recipients did not prevent the effect of C. granulosum. Treatment i.p. or i.v. with this bacterium significantly decreased the number of lung metastases from i.v.-injected fibrosarcoma cells, even if the cells were injected 3 to 4 months later. The magnitude of this effect varied with the dose and frequency of injection of C. granulosum and C. parvum.
我们研究了单次和多次注射颗粒棒状杆菌对C3Hf/Bu小鼠淋巴结和脾脏重量及组织学、外周白细胞计数、外周血淋巴细胞反应、淋巴结和脾细胞对植物血凝素和商陆有丝分裂原的反应、皮肤同种异体移植存活以及同基因纤维肉瘤肺转移的影响。在一些抗肿瘤活性研究中使用了短小棒状杆菌。单次和多次腹腔注射颗粒棒状杆菌可使淋巴结和脾脏重量显著增加，淋巴结在第7天达到肿大峰值，脾脏在第16天达到肿大峰值。组织学上，肿大器官中有核细胞广泛增殖。颗粒棒状杆菌未改变白细胞总数，但导致暂时性淋巴细胞减少。一般来说，血液淋巴细胞和脾细胞对植物血凝素和商陆有丝分裂原的体外反应降低。小剂量（0.025mg）颗粒棒状杆菌可增加淋巴结细胞对植物血凝素的反应，单次大剂量（0.5mg）无改变，多次剂量则降低。所有处理均可增加淋巴结细胞对商陆有丝分裂原的反应。这些对有丝分裂原反应的变化在治疗后约2个月内可检测到。在移植前而非移植后静脉注射0.1或0.25mg颗粒棒状杆菌可显著延长移植的BALB/c皮肤的存活时间。较小剂量的这种细菌无效。皮肤移植受体的脾切除术不能阻止颗粒棒状杆菌的作用。腹腔或静脉注射这种细菌可显著减少静脉注射纤维肉瘤细胞后的肺转移数量，即使细胞在3至4个月后注射。这种作用的程度随颗粒棒状杆菌和短小棒状杆菌的剂量和注射频率而变化。
