通过亲和层析法纯化多核苷酸磷酸化酶及纯化酶的一些性质
Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes.
作者信息
Smith J C, Eaton M A
出版信息
Nucleic Acids Res. 1974 Dec;1(12):1763-73. doi: 10.1093/nar/1.12.1763.
Abstract
A method is described for the preparation of p-aminophenyl oligo(dT)-Sepharose. This matrix has been used for the purification of polynucleotide phosphorylase from both E.coli and B.stearothermophilus. The effects of temperature and pH on the binding of the different enzymes to the matrix have been investigated. B.stearothermophilus isolated by affinity chromatography may be useful in selectively removing the polyA tract on the 3'-end of mRNA's.
摘要
描述了一种制备对氨基苯基寡聚（dT）-琼脂糖的方法。该基质已用于从大肠杆菌和嗜热脂肪芽孢杆菌中纯化多核苷酸磷酸化酶。研究了温度和pH对不同酶与基质结合的影响。通过亲和色谱法分离的嗜热脂肪芽孢杆菌可能有助于选择性去除mRNA 3'末端的聚A序列。
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