通过亲和层析法纯化多核苷酸磷酸化酶及纯化酶的一些性质
Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes.
作者信息
Smith J C, Eaton M A
出版信息
Nucleic Acids Res. 1974 Dec;1(12):1763-73. doi: 10.1093/nar/1.12.1763.
Abstract
A method is described for the preparation of p-aminophenyl oligo(dT)-Sepharose. This matrix has been used for the purification of polynucleotide phosphorylase from both E.coli and B.stearothermophilus. The effects of temperature and pH on the binding of the different enzymes to the matrix have been investigated. B.stearothermophilus isolated by affinity chromatography may be useful in selectively removing the polyA tract on the 3'-end of mRNA's.
摘要
描述了一种制备对氨基苯基寡聚（dT）-琼脂糖的方法。该基质已用于从大肠杆菌和嗜热脂肪芽孢杆菌中纯化多核苷酸磷酸化酶。研究了温度和pH对不同酶与基质结合的影响。通过亲和色谱法分离的嗜热脂肪芽孢杆菌可能有助于选择性去除mRNA 3'末端的聚A序列。
相似文献
1
Purification of polynucleotide phosphorylase by affinity chromatography and some properties of the purified enzymes.通过亲和层析法纯化多核苷酸磷酸化酶及纯化酶的一些性质
Nucleic Acids Res. 1974 Dec;1(12):1763-73. doi: 10.1093/nar/1.12.1763.
2
[Isolation and some properties of polynucleotide phosphorylase from E. coli].[大肠杆菌多核苷酸磷酸化酶的分离及某些性质]
Biokhimiia. 1981 May;46(5):802-8.
3
Thermostable polynucleotide phosphorylases from Bacillus stearothermophilus and Thermus aquaticus.来自嗜热脂肪芽孢杆菌和嗜热水生栖热菌的热稳定多核苷酸磷酸化酶。
Nucleic Acids Res. 1976 Jan;3(1):219-29. doi: 10.1093/nar/3.1.219.
4
Blue-dextran--Sepharose affinity chromatography: recognition of a polynucleotide binding site of a protein.蓝色葡聚糖-琼脂糖亲和层析：蛋白质多核苷酸结合位点的识别
Eur J Biochem. 1978 Jan 16;82(2):355-62. doi: 10.1111/j.1432-1033.1978.tb12030.x.
5
Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase.多核苷酸磷酸化酶的结构-功能关系研究：一种蛋白水解降解的多核苷酸磷酸化酶模型
Nucleic Acids Res. 1976 Nov;3(11):3015-24. doi: 10.1093/nar/3.11.3015.
6
Purification and characterization of polynucleotide phosphorylase from Escherichia coli. Probe for the analysis of 3' sequences of RNA.
J Biol Chem. 1977 Oct 10;252(19):6885-8.
7
Polynucleotide synthetase of E. coli: an enzyme complex having polynucleotide phosphorylase as apoenzyme.大肠杆菌的多核苷酸合成酶：一种以多核苷酸磷酸化酶为辅酶的酶复合物。
Biochim Biophys Acta. 1981 Oct 27;655(3):307-22. doi: 10.1016/0005-2787(81)90040-x.
8
Enzymatic synthesis of deoxyribo-oligonucleotides of defined sequence. Properties of the enzyme.特定序列脱氧核糖寡核苷酸的酶促合成。该酶的性质。
Nucleic Acids Res. 1974 Dec;1(12):1631-47. doi: 10.1093/nar/1.12.1631.
9
The purification of polynucleotide phosphorylase from Thermus aquaticus by the use of heparin-sepharose 4B affinity chromatography.
Acta Biochim Biophys Acad Sci Hung. 1981;16(3-4):135-44.
10
[Determination of the activity of a polynucleotide phosphorylase preparation from E. coli].[大肠杆菌多核苷酸磷酸化酶制剂活性的测定]
Prikl Biokhim Mikrobiol. 1983 Jul-Aug;19(4):555-9.
本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
J Biol Chem. 1970 Jun;245(12):3059-65.
3
Deoxyadenosine diphosphate as a substrate and inhibitor of polynucleotide phosphorylase of Micrococcus luteus. I. Deoxyadenosine diphosphate as a substrate for polymerization and the exchange reaction with inorganic 32 P.二磷酸脱氧腺苷作为藤黄微球菌多核苷酸磷酸化酶的底物和抑制剂。I. 二磷酸脱氧腺苷作为聚合反应的底物以及与无机32P的交换反应
J Biol Chem. 1971 Dec 25;246(24):7486-96.
4
Poly(5-chlorocytidylic acid).聚（5-氯胞苷酸）
Biochemistry. 1972 Aug 15;11(17):3162-7. doi: 10.1021/bi00767a004.
5
Kinetics of polymerization and phosphorolysis reactions of Escherichia coli polynucleotide phosphorylase. Evidence for multiple binding of polynucleotide in phosphorolysis.大肠杆菌多核苷酸磷酸化酶的聚合反应和磷酸解反应动力学。磷酸解中多核苷酸多重结合的证据。
Eur J Biochem. 1970 Jun;14(2):222-31. doi: 10.1111/j.1432-1033.1970.tb00281.x.
6
Purification and properties of polynucleotide phosphorylase from Escherichia coli.
J Biol Chem. 1968 Jan 25;243(2):231-40.
7
Precipitation of nucleic acids with polyethyleneimine and the chromatography of nucleic acids and proteins on immobilised polyethyleneimine.用聚乙烯亚胺沉淀核酸以及核酸和蛋白质在固定化聚乙烯亚胺上的色谱分析
Biochim Biophys Acta. 1973 Apr 21;308(7):41-52. doi: 10.1016/0005-2787(73)90120-2.
8
Separation of Escherichia coli ribonucleases on a DNA agarose column and the identification of an RNase H activity.在DNA琼脂糖柱上分离大肠杆菌核糖核酸酶并鉴定核糖核酸酶H活性。
Biochem Biophys Res Commun. 1972 Dec 4;49(5):1307-15. doi: 10.1016/0006-291x(72)90609-2.
9
Affinity chromatography.亲和层析
Annu Rev Biochem. 1971;40:259-78. doi: 10.1146/annurev.bi.40.070171.001355.
10
Kinetic analysis of the phosphorolysis of oligonucleotides by polynucleotide phosphorylase.多核苷酸磷酸化酶对寡核苷酸磷酸解作用的动力学分析。
J Biol Chem. 1970 Mar 10;245(5):995-1004.
Zotero 插件