多核苷酸磷酸化酶的结构-功能关系研究：一种蛋白水解降解的多核苷酸磷酸化酶模型
Study on the structure-function relationship of polynucleotide phosphorylase: model of a proteolytic degraded polynucleotide phosphorylase.
作者信息
Guissani A, Portier C
出版信息
Nucleic Acids Res. 1976 Nov;3(11):3015-24. doi: 10.1093/nar/3.11.3015.
Abstract
It is already known that modification of E. coli polynucleotide phosphorylase by endogenous proteolysis induces drastic changes in both phosphorolysis and polymerisation reactions. The structural parameters of the proteolysed polynucleotide phosphorylase are described. The phosphorolysis of polynucleotide, which is quite progressive for the native enzyme, is shown to be only partially progressive for the degraded enzyme, owing to the loss of polymer attachment sites.
摘要
已知内源性蛋白水解对大肠杆菌多核苷酸磷酸化酶的修饰会在磷酸解和聚合反应中引发剧烈变化。文中描述了蛋白水解后的多核苷酸磷酸化酶的结构参数。对于天然酶而言相当渐进的多核苷酸磷酸解，对于降解后的酶仅部分渐进，这是由于聚合物附着位点的丧失。
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