用（³H）色氨酸孵育豚鼠胰岛后标记蛋白质的放射自显影定位。
Radioautographic localization of labelled proteins after incubation of guinea-pig islets of Langerhans with (3H)tryptophan.
作者信息
Howell S L, Hellerström C, Whitfield M
出版信息
Biochem J. 1974 Apr;140(1):22-3. doi: 10.1042/bj1400022.
Abstract
An analysis was made by electron-microscopic radioautography of the distribution of silver grains over storage granules of A(2) and B cells after incubation of isolated guinea-pig islets of Langerhans for 17h in the presence of [(3)H]tryptophan. A significant concentration of labelled proteins was present in the A(2) cell, but not in the B-cell granules.
摘要
在存在[³H]色氨酸的情况下，将分离的豚鼠胰岛朗格汉斯岛培养17小时后，通过电子显微镜放射自显影分析了银颗粒在A(2)细胞和B细胞储存颗粒上的分布。在A(2)细胞中存在大量标记蛋白，但在B细胞颗粒中不存在。
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