大肠杆菌核糖体蛋白的拓扑结构。巯基的反应顺序。
Topography of Escherichia coli ribosomal proteins. The order of reactivity of thiol groups.
作者信息
Bakardjieva A, Crichton R R
出版信息
Biochem J. 1974 Dec;143(3):599-606. doi: 10.1042/bj1430599.
Abstract
- 30S and 50S ribosomal subunits of Escherichia coli were treated with N-[2,3-(14)C]-ethylmaleimide and iodo[(14)C]acetamide. 2. The proteins in the native subunits which reacted with the reagents were S1,double dagger S2, S12, S13, S18, S21, L2, L5, L6, L10, L11, L15, L17, L20, L26+28 and L27. 3. Several proteins, such as S1, S12, S14, S18, L2, L6, L10, L11 and either L26 or 28, had thiol groups in an oxidized form and reacted to a greater extent after reduction with beta-mercaptoethanol or dithiothreitol. 4. The total number of thiol groups in 30S and 50S subunits was determined as 16-17 and 26-27 respectively. The total number of thiol groups in each ribosomal protein was also determined. 5. The reaction of 30S and 50S subunits with iodoacetamide under several different conditions established the order of reactivity of thiol groups.
摘要
- 用N-[2,3-(14)C]-乙基马来酰亚胺和碘[(14)C]乙酰胺处理大肠杆菌的30S和50S核糖体亚基。2. 天然亚基中与试剂反应的蛋白质有S1、双剑号S2、S12、S13、S18、S21、L2、L5、L6、L10、L11、L15、L17、L20、L26+28和L27。3. 几种蛋白质，如S1、S12、S14、S18、L2、L6、L10、L11以及L26或28，具有氧化形式的巯基，在用β-巯基乙醇或二硫苏糖醇还原后反应程度更大。4. 30S和50S亚基中巯基的总数分别确定为16 - 17个和26 - 27个。还确定了每个核糖体蛋白质中巯基的总数。5. 在几种不同条件下30S和50S亚基与碘乙酰胺的反应确定了巯基的反应活性顺序。
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