兔肌肉磷酸丙糖异构酶的胰蛋白酶肽段
The tryptic peptides of rabbit muscle triose phosphate isomerase.
作者信息
Corran P H, Waley S G
出版信息
Biochem J. 1974 Apr;139(1):1-10. doi: 10.1042/bj1390001.
Abstract
- The peptides obtained by tryptic digestion of S-[(14)C]carboxymethylated rabbit muscle triose phosphate isomerase have been studied. 2. The first step in the fractionation of the tryptic digest was gel filtration on coupled columns of Sephadex G-25 and G-50. Further fractionation was carried out by paper electrophoresis and paper chromatography. 3. The digest contained 26 peptides and three free amino acids. The sizes of the peptides ranged from two to 29 residues. 4. The sequences of the peptides have been determined. 5. The length of the polypeptide chains is about 250 amino acid residues. 6. The variant sequences encountered were due to partial deamidation; this may be one of the reasons for multiple forms of the enzyme. 7. The chicken and rabbit enzymes are compared. 8. Detailed evidence for the sequences of the tryptic peptides has been deposited as Supplementary Publication SUP 50024 at the British Library, Lending Division (formerly the National Lending Library for Science and Technology), Boston Spa, Yorks. LS23 7BQ, U.K., from whom copies can be obtained on the terms given in Biochem. J. (1973) 131, 5.
摘要
- 对经S-[(14)C]羧甲基化的兔肌肉磷酸丙糖异构酶进行胰蛋白酶消化所得到的肽段进行了研究。2. 胰蛋白酶消化产物分级分离的第一步是在Sephadex G-25和G-50偶联柱上进行凝胶过滤。进一步的分级分离通过纸电泳和纸色谱法进行。3. 消化产物含有26个肽段和三种游离氨基酸。肽段的大小范围从2个到29个残基。4. 已确定了肽段的序列。5. 多肽链的长度约为250个氨基酸残基。6. 所遇到的变异序列是由于部分脱酰胺作用；这可能是该酶存在多种形式的原因之一。7. 对鸡和兔的酶进行了比较。8. 胰蛋白酶肽段序列的详细证据已作为补充出版物SUP 50024存放在英国利兹市波士顿温泉约克郡LS23 7BQ的大英图书馆出借部（原国家科学技术出借图书馆），可按《生物化学杂志》（1973年）第131卷第5期所规定的条件从该处获取复印件。
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