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兔骨骼肌肌浆网钙离子转运三磷酸腺苷酶的一级结构。羧甲基化蛋白经α-胰凝乳蛋白酶消化后的可溶性肽段。

Primary structure of the calcium ion-transporting adenosine triphosphatase of rabbit skeletal sarcoplasmic reticulum. Soluble peptides from the alpha-chymotryptic digest of the carboxymethylated protein.

作者信息

Allen G

出版信息

Biochem J. 1980 Jun 1;187(3):565-75. doi: 10.1042/bj1870565.

Abstract

The isolation of the soluble peptides from the chymotryptic digest of the calcium-transporting ATPase of rabbit skeletal sarcoplasmic reticulum is described. These peptides were partially sequenced and the information obtained was used to align tryptic peptides of the protein and to confirm sequences within the tryptic peptides. Details of the isolation of some peptides and the amino acid analyses of the peptides are given in Supplementary Publication SUP 50103 (10 pages), which has been deposited with the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1978) 169, 5.

摘要

本文描述了从兔骨骼肌肌浆网钙转运ATP酶的胰凝乳蛋白酶消化物中分离可溶性肽的过程。对这些肽进行了部分测序,并利用所获得的信息来比对该蛋白质的胰蛋白酶肽段,并确认胰蛋白酶肽段内的序列。一些肽的分离细节和肽的氨基酸分析见补充出版物SUP 50103(共10页),该出版物已存放于英国西约克郡韦瑟比市波士顿温泉镇的大英图书馆出借部,邮编为LS23 7BQ,可按《生物化学杂志》(1978年)第169卷第5页所示条件从该处获取复印件。

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