Antilymphocyte antibody purified by immunoabsorption and elution.

A comparison has been made of several techniques for the preparation from antilymphocyte serum of pure antilymphocyte antibody by absorption on, and elution from mouse thymocytes. The best yield was obtained by a batch method using cells fixed in 0·1% formaldehyde as immunoabsorbent followed by elution with 0·12 M citrate buffer at pH 3·0. The antilymphocyte activity of eluates was studied by determination of membrane immunofluorescence titres and by the neutralization of the local renal xenogeneic graft-versus-host reaction. These tests showed that an IgG eluate of a rabbit antilymphocyte serum was nineteen times more active than the same weight of the whole IgG present in the parent serum.