[人凝血因子VIII的解离及VIII-C和VIII-AG片段的重组]
[Dissociation of human factor VIII and recombination of the fragments VIII-C and VIII-AG].
作者信息
Tran T H, Bounameaux H, Marbet G A, Duckert F
出版信息
Schweiz Med Wochenschr. 1979 Jul 14;109(28):1029-34.
PMID:462170
Abstract
Human factor VIII has been dissociated into fragment VIII-C and fragment VIII-AG in the presence of calcium chloride (0.25 M). After removing the calcium, recombination of the molecule is obtained. Fragment VIII-C can be activated by thrombin in a similar manner to the coagulant activity of whole factor VIII. This activation is calcium-dependent. In rabbits immunized against purified F VIII, VIII-C and VIII-AG specific antisera against these three entities were obtained.
摘要
在氯化钙（0.25M）存在的情况下，人凝血因子VIII已被解离为VIII - C片段和VIII - AG片段。去除钙离子后，可实现该分子的重组。VIII - C片段可被凝血酶以与全因子VIII的凝血活性类似的方式激活。这种激活是钙依赖性的。在针对纯化的F VIII免疫的兔子中，获得了针对这三种实体的VIII - C和VIII - AG特异性抗血清。
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