Fan D P, Beckman M M, Cunningham W P
J Bacteriol. 1972 Mar;109(3):1247-57. doi: 10.1128/jb.109.3.1247-1257.1972.
The growth of Bacillus subtilis mutant betaA177 can be inhibited under special conditions in which not enough autolytic enzymes are produced for optimal growth. Electron microscopy studies show that during growth inhibition there is localized thickening of the cell wall at positions where cells bend. A model is proposed to explain this result. Rapid growth can be restored by adding lysozyme or a B. subtilis autolysin mixture to a growth-inhibited betaA177 culture. Such addition reduces the localized wall thickening and causes other changes in surface morphology which are described and discussed. Septum formation seems to be relatively less inhibited than cell elongation when lytic enzyme levels are reduced. Measurements were made demonstrating that walls at ends of cells are morphologically different from walls at sides of cells in cultures of betaA177 growing at 51 C.
枯草芽孢杆菌突变体betaA177在特殊条件下生长会受到抑制，即产生的自溶酶不足以实现最佳生长。电子显微镜研究表明，在生长抑制期间，细胞壁在细胞弯曲的位置出现局部增厚。本文提出了一个模型来解释这一结果。向生长受抑制的betaA177培养物中添加溶菌酶或枯草芽孢杆菌自溶酶混合物，可以恢复快速生长。这种添加减少了局部细胞壁增厚，并导致表面形态发生其他变化，本文对此进行了描述和讨论。当裂解酶水平降低时，隔膜形成似乎比细胞伸长受到的抑制相对较小。测量结果表明，在51℃下生长的betaA177培养物中，细胞末端的细胞壁与细胞侧面的细胞壁在形态上有所不同。
