Forsberg C W, Wyrick P B, Ward J B, Rogers H J
J Bacteriol. 1973 Feb;113(2):969-84. doi: 10.1128/jb.113.2.969-984.1973.
Two very poorly lytic mutants of Bacillus licheniformis 6346 that had no teichuronic acid or glucose in their walls were phosphoglucomutase deficient. The walls of the mutants were less autolytic, and the lesion in the phosphoglucomutase gene and the formation of lytic amidase seemed to be interrelated. When phosphoglucomutase was regained or the effects of the deficiency were circumvented by the presence of galactose in the medium, the lytic enzyme was partially regained. When subjected to growth limitation by the supply of inorganic phosphate, the mutants ceased to make teichoic acid, and their walls contained a greatly increased proportion of mucopeptide. Under these conditions they formed irregular spheres which changed back to rods when inorganic phosphate was supplied. Both wall and protein synthesis were necessary for the changes in morphology. An intermediate crescent-shaped cell was formed in the change from sphere to a rod. The possible relationship of this morphological change to the distribution of biosynthetic sites is discussed.
地衣芽孢杆菌6346的两个裂解能力非常弱的突变体，其细胞壁中没有磷壁酸或葡萄糖，它们缺乏磷酸葡萄糖变位酶。这些突变体的细胞壁自溶能力较弱，磷酸葡萄糖变位酶基因的损伤与裂解酰胺酶的形成似乎相互关联。当磷酸葡萄糖变位酶恢复，或者培养基中半乳糖的存在规避了缺陷的影响时，裂解酶部分恢复。当通过供应无机磷酸盐使生长受到限制时，这些突变体停止合成磷壁酸，其细胞壁中粘肽的比例大幅增加。在这些条件下，它们形成不规则球体，当供应无机磷酸盐时又变回杆状。形态变化需要细胞壁和蛋白质合成。从球体变为杆状的过程中形成了中间新月形细胞。讨论了这种形态变化与生物合成位点分布的可能关系。
