Rubio N, Lopez R
Appl Microbiol. 1972 Feb;23(2):211-3. doi: 10.1128/am.23.2.211-213.1972.
A procedure is described for obtaining large quantities of purified endotoxin of Pseudomonas aeruginosa by using Diaflo ultrafiltration. This method allowed us to isolate from the protein-lipopolysaccharide complex two low-molecular-weight substances which do not play any antigenic role. It provides a useful tool for immunological purposes.
描述了一种通过使用Diaflo超滤获得大量纯化铜绿假单胞菌内毒素的方法。该方法使我们能够从蛋白质 - 脂多糖复合物中分离出两种不具有任何抗原作用的低分子量物质。它为免疫学目的提供了一种有用的工具。