用于痘苗病毒检测的免疫荧光细胞计数法与蚀斑法的比较。
Comparison of the immunofluorescent-cell counting and plaque methods for the assay of vaccinia virus.
作者信息
Ibrahim A L, Loh P C
出版信息
Appl Microbiol. 1972 Feb;23(2):214-7. doi: 10.1128/am.23.2.214-217.1972.
Abstract
The immunofluorescent-cell counting method was compared with the agarand liquid-overlay plaque techniques for the assay of vaccinia virus. In addition to being as quantitative, reproducible, and simple to use as the two plaque techniques, the immunofluorescent assay method was found to be more sensitive, rapid, and specific.
摘要
将免疫荧光细胞计数法与用于痘苗病毒测定的琼脂覆盖法和液体覆盖蚀斑技术进行了比较。结果发现，免疫荧光测定法除了具有与两种蚀斑技术一样的定量、可重复和易于使用的特点外，还更灵敏、快速且特异。
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2
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Nature. 1962 Jan 13;193:150-1. doi: 10.1038/193150a0.
3
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Virology. 1960 Apr;10:466-82. doi: 10.1016/0042-6822(60)90130-6.
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