Ehrhard H B, Pine L
Appl Microbiol. 1972 Feb;23(2):250-61. doi: 10.1128/am.23.2.250-261.1972.
Stagnant culture methods have permitted only limited physiological studies of the production of H and M antigens by Histoplasma capsulatum because, with such methods, antigen production is uncontrolled. In this investigation, a shake culture method was used to convert yeast-phase inoculum to mycelialphase growth at 25 C. Results strongly suggest that the release of H and M antigens relates to autolysis of the cells. Among the factors influencing production of H and M antigens under shaking conditions, choice of strain was the most important. Alterations of carbon or nitrogen source or variations in amino acid to carbohydrate ratios had limited influence on antigen production. With a strain that produced both H and M antigens, however, proportions of titers of M to H antigens could be made to vary considerably by changes in the medium, the pH, and the temperature. Results suggest that the source of M antigen during autolysis is enzymatic dissolution of the cell wall. The source of H antigen is more obscure. Production of both antigens may be differentially controlled under conditions of good reproducibility by a correct choice of strain and manipulation of culture medium.
停滞培养法仅允许对荚膜组织胞浆菌产生H和M抗原的生理过程进行有限的研究，因为采用这种方法时，抗原的产生是不受控制的。在本研究中，采用振荡培养法在25℃下将酵母相接种物转化为菌丝相生长。结果有力地表明，H和M抗原的释放与细胞的自溶有关。在振荡条件下影响H和M抗原产生的因素中，菌株的选择是最重要的。碳源或氮源的改变或氨基酸与碳水化合物比例的变化对抗原产生的影响有限。然而，对于一种能同时产生H和M抗原的菌株，通过改变培养基、pH值和温度，M与H抗原的效价比例会有很大变化。结果表明，自溶过程中M抗原的来源是细胞壁的酶解。H抗原的来源则更为模糊。通过正确选择菌株和控制培养基，在良好的重复性条件下，两种抗原的产生可能受到不同的调控。
