WIGGINS G L, SCHUBERT J H
J Bacteriol. 1965 Mar;89(3):589-96. doi: 10.1128/jb.89.3.589-596.1965.
Wiggins, Geraldine L. (Communicable Disease Center, Atlanta, Ga.), and Joseph H. Schubert. Relationship of histoplasmin agar-gel bands and complement-fixation titers in histoplasmosis. J. Bacteriol. 89:589-596. 1965.-Culture filtrates of various strains of Histoplasma capsulatum were studied with sera from patients with histoplasmosis, by use of the agar-gel and complement-fixation tests. It was found that the filtrates represented three types: those containing H and M components, those with H only, and those with neither H nor M. The same strain of H. capsulatum often produced culture filtrates of more than one type. Complement fixation showed that only the filtrates having both the H and M components were sufficiently sensitive as diagnostic antigens to detect the antibodies in histoplasmosis. H and M fractions prepared by ion-exchange chromatography were found to produce more than one band; therefore, it was concluded that the available fractions were not pure. The formation and identification of bands were affected by the concentration of the antigen and the arrangement of the wells containing the antigen and serum. The optimal concentration of the antigen varied, depending on the position of these reagents. The method found most practical for the identification of bands in unknown sera consisted of selecting the optimal concentration of the components with a box titration, by use of antigen having both the H and M components, placing known positive sera in wells adjacent to the unknown sera, and studying the sera for lines of identity. The M band was usually closest to the antigen well, and the H was nearest the serum well. These bands could be reversed by dilution, adjusting the H antibodies so that they equalled or exceeded the M antibodies. One human serum in which the bands were reversed showed the H bands at a higher serum dilution than that obtained with the M band. Patients with culturally proven histoplasmosis had the M band in their serum regardless of the stage of disease or length of duration; only 4 of 10 patients in the active stage of histoplasmosis also had the H band. The low prevalence of the H band would eliminate it as a diagnostic tool for detecting the active stage of the disease. Other bands obtained with histoplasmin antigen in the sera from proven cases were of no assistance in determining the clinical status of the patient.
威金斯，杰拉尔丁·L.（佐治亚州亚特兰大市传染病中心）和约瑟夫·H.舒伯特。组织胞浆菌病中组织胞浆菌素琼脂凝胶带与补体结合滴度的关系。《细菌学杂志》89:589 - 596。1965年。——利用琼脂凝胶试验和补体结合试验，用组织胞浆菌病患者的血清研究了各种荚膜组织胞浆菌菌株的培养滤液。发现滤液有三种类型：含有H和M成分的、仅含H的以及既不含H也不含M的。同一株荚膜组织胞浆菌常常产生不止一种类型的培养滤液。补体结合试验表明，只有同时含有H和M成分的滤液作为诊断抗原足够敏感，能够检测出组织胞浆菌病中的抗体。通过离子交换色谱法制备的H和M组分产生了不止一条带；因此，得出结论，可用的组分不纯。带的形成和鉴定受抗原浓度以及含抗原和血清的孔的排列影响。抗原的最佳浓度因这些试剂的位置而异。发现最实用的鉴定未知血清中带的方法包括：通过使用同时含有H和M成分的抗原进行方格滴定来选择组分的最佳浓度，在未知血清相邻的孔中放置已知阳性血清，并研究血清中的同一线。M带通常最靠近抗原孔，H带最靠近血清孔。通过稀释可以使这些带颠倒，调整H抗体使其等于或超过M抗体。有一份人血清中的带颠倒了，其H带在比M带更高的血清稀释度下出现。经培养证实患有组织胞浆菌病的患者，无论疾病阶段或病程长短，其血清中都有M带；在组织胞浆菌病活动期的10名患者中只有4名同时也有H带。H带的低发生率使其不能作为检测疾病活动期的诊断工具。在确诊病例的血清中用组织胞浆菌素抗原获得的其他带无助于确定患者的临床状况。
