金鱼对蛋白质免疫原和半抗原决定簇的抗体反应。
Antibody response of goldfish to a protein immunogen and to haptenic determinants.
作者信息
Everhart D L
出版信息
Immunology. 1972 Mar;22(3):503-7.
The serum antibody response of goldfish () to protein immunogen (bovine serum albumin, BSA) and haptenic determinants was studied. The goldfish antibody response was compared with rabbit antibody response to similar antigens. The goldfish received BSA only or BSA with one of three haptens coupled to it. Arsanilic acid, sulphanilic acid and para-amino-benzoic acid were used as haptens. Precipitation and passive haemagglutination were used to demonstrate the presence of antibody. Only one size of antibody could be demonstrated to all antigens (13.2S). Two populations of antibody could be demonstrated by the use of electrophoresis, and both populations of antibody reacted with BSA in fish receiving only BSA. In fish receiving BSA-hapten, the BSA antibody was found in the slower migrating population, while the haptenic antibody was found in the faster migrating population. The fish antibodies to all immunogens were poor precipitins, but gave titres similar to rabbit antibody when tested by passive haemagglutination. The specificity of fish protein and haptenic antibody is similar to rabbit antibody.
研究了金鱼（）对蛋白质免疫原（牛血清白蛋白，BSA）和半抗原决定簇的血清抗体反应。将金鱼的抗体反应与兔子对类似抗原的抗体反应进行了比较。金鱼仅接受BSA或与三种半抗原之一偶联的BSA。对氨基苯胂酸、对氨基苯磺酸和对氨基苯甲酸用作半抗原。采用沉淀法和被动血凝法检测抗体的存在。对所有抗原仅能检测到一种大小的抗体（13.2S）。通过电泳可检测到两类抗体，且在仅接受BSA的鱼中，这两类抗体均与BSA发生反应。在接受BSA-半抗原的鱼中，BSA抗体存在于迁移较慢的组分中，而半抗原抗体存在于迁移较快的组分中。鱼对所有免疫原产生的抗体沉淀反应较差，但通过被动血凝法检测时，其滴度与兔子抗体相似。鱼的蛋白质抗体和半抗原抗体的特异性与兔子抗体相似。
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