Desvaux F X, Charlemagne J
Immunology. 1981 Aug;43(4):755-62.
Anti-red blood cells (RBC) and anti-hapten antibody synthesis were studied in the goldfish, Carassius auratus. Spontaneous haemagglutination titres were found against all the antigens tested. A weak secondary response was observed in RBC-primed fish boosted during the end-phase of the primary antibody production. However, when the second antigenic challenge was performed during the early exponential phase of a primary stimulation, an important amplified response was obtained. The antibody production and immunological memory can be dissociated: no antibody synthesis occurred in glutaraldehyde-fixed RBC (F-RBC) primed was obtained when untreated or F-RBC were given to F-RBC primed animals. The amplified response to sheep red blood cells (SRBC) was significantly inhibited when fish were primed with a mixture of SRBC and Xenopus red blood cells (XRBC), demonstrating an antigenic competition phenomenon. Studies on anti-trinitrobenzene responses confirm the efficiency of E. coli lipopolysaccharide as a carrier for fish anti-hapten immunization. The kinetics and regulation of antibody synthesis in fish are discussed in relation to the described results.
在金鱼（Carassius auratus）中研究了抗红细胞（RBC）和抗半抗原抗体的合成。发现针对所有测试抗原均有自发血凝滴度。在初次抗体产生末期接受加强免疫的红细胞致敏鱼中观察到较弱的二次反应。然而，当在初次刺激的早期指数期进行第二次抗原攻击时，获得了重要的放大反应。抗体产生和免疫记忆可以分离：当将未处理的或戊二醛固定的红细胞（F-RBC）给予F-RBC致敏动物时，未获得F-RBC致敏的抗体合成。当用绵羊红细胞（SRBC）和非洲爪蟾红细胞（XRBC）的混合物对鱼进行致敏时，对SRBC的放大反应受到显著抑制，证明了抗原竞争现象。抗三硝基苯反应的研究证实了大肠杆菌脂多糖作为鱼类抗半抗原免疫载体的有效性。结合所述结果讨论了鱼类抗体合成的动力学和调节。
