Judd B H, Shen M W, Kaufman T C
Genetics. 1972 May;71(1):139-56. doi: 10.1093/genetics/71.1.139.
An average size chromomere of the polytene X chromosome of Drosophila melanogaster contains enough DNA in each haploid equivalent strand to code for 30 genes, each 1,000 nucleotides long. We have attempted to learn about the organization of chromosomes by asking how many functional units can be localized within a chromomere. This was done by 1) recovery of mutants representative of every cistron in the 3A2-3C2 region; 2) the characterization of the function of each mutant type and grouping by complementation tests; 3) the determination of the genetic and cytological position of each cistron by recombination and deletion mapping. The data clearly show one functional group per chromomere. It is postulated that a chromomere is one cistron within which much of the DNA is regulatory in function.
黑腹果蝇多线X染色体的一个平均大小的染色粒，在每条单倍体等效链中所含的DNA足以编码30个基因，每个基因长1000个核苷酸。我们试图通过询问在一个染色粒内可以定位多少个功能单位来了解染色体的组织方式。这是通过以下方式完成的：1）回收代表3A2 - 3C2区域中每个顺反子的突变体；2）对每种突变体类型的功能进行表征，并通过互补试验进行分组；3）通过重组和缺失作图确定每个顺反子的遗传和细胞学位置。数据清楚地表明每个染色粒有一个功能组。据推测，一个染色粒就是一个顺反子，其中大部分DNA具有调控功能。
