肝炎相关抗原检测方法的评估
Evaluation of assay methods for hepatitis-associated antigen.
作者信息
Kissling R E, Barker L F
出版信息
Appl Microbiol. 1972 Jun;23(6):1037-46. doi: 10.1128/am.23.6.1037-1046.1972.
Assay methods for hepatitis-associated antigen (HAA) were evaluated for sensitivity, or reproducibility, or both in a series of three trials in which both research and service-oriented laboratories participated. Agar-gel diffusion (AGD) methods were found to be the least sensitive and reproducible of the commonly employed assay methods. Complement fixation (CF) tests were consistently more sensitive than either AGD or counterelectrophoresis (CEP) methods for detection of HAA. With judicious choice of the antibody reagent, sensitivity of CEP techniques was equivalent to CF methods of HAA detection. None of the three major assay methods (AGD, CEP, or CF) compared in this study were capable of consistently detecting HAA when it was present in relatively low concentrations in human serum.
在一系列三项试验中，对肝炎相关抗原（HAA）的检测方法进行了敏感性、可重复性或两者的评估，研究型实验室和服务型实验室均参与其中。琼脂凝胶扩散（AGD）方法被发现是常用检测方法中最不敏感且重复性最差的。补体结合（CF）试验在检测HAA方面始终比AGD或对流电泳（CEP）方法更敏感。通过明智地选择抗体试剂，CEP技术的敏感性与检测HAA的CF方法相当。在本研究中比较的三种主要检测方法（AGD、CEP或CF），当HAA在人血清中以相对较低浓度存在时，均无法始终检测到它。
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