Peterson D A, Froesner G G, Deinhardt F W
Appl Microbiol. 1973 Sep;26(3):376-80. doi: 10.1128/am.26.3.376-380.1973.
Sensitivity and specificity of passive hemagglutination (RCA), solid phase radioimmunoassay (RIA), and immunoelectroosmophoresis (IEOP) were compared under experimental and clinical conditions. In dilution experiments with sera containing hepatitis B antigen (HB Ag) of known subtypes, the sensitivity for an ad subtype serum was RIA (1), RCA (1/2), IEOP (1/256) and for an ay subtype serum RCA (1), RIA (1/8), IEOP (1/128). An evaluation of the National Institutes of Health, Division of Biologics Standards test panel number 2 demonstrated HB Ag in 34 of 60 samples by RIA, in 33 by RCA, and in 25 by IEOP. HB Ag was detected in 57.5% of 200 outpatients with a tentative diagnosis of hepatitis by RIA, in 54% by RCA, and in 42.5% by IEOP. In 1,661 volunteer blood donors, 13 (0.78%) were "positive" for HB Ag by RIA, 11 (0.66%) by RCA, and 3 (0.18%) by IEOP. However, absorption experiments indicated that at least six of the above RIA positive and five of the RCA positive sera exhibited nonspecific positive reactions.
在实验和临床条件下，比较了被动血凝反应（RCA）、固相放射免疫测定（RIA）和免疫电渗电泳（IEOP）的敏感性和特异性。在对含有已知亚型乙肝抗原（HB Ag）的血清进行稀释实验时，对于ad亚型血清，RIA的敏感性为1，RCA为1/2，IEOP为1/256；对于ay亚型血清，RCA的敏感性为1，RIA为1/8，IEOP为1/128。对美国国立卫生研究院生物制品标准司第2号测试样本组的评估显示，用RIA法在60个样本中的34个检测到HB Ag，用RCA法在33个样本中检测到，用IEOP法在25个样本中检测到。在200名初步诊断为肝炎的门诊患者中，用RIA法在57.5%的患者中检测到HB Ag；用RCA法在54%的患者中检测到；用IEOP法在42.5%的患者中检测到。在1661名志愿献血者中，用RIA法检测到13人（0.78%）HB Ag呈“阳性”，用RCA法检测到11人（0.66%）呈“阳性”，用IEOP法检测到3人（0.18%）呈“阳性”。然而，吸收实验表明，上述RIA法检测为阳性的血清中至少有6份以及RCA法检测为阳性的血清中至少有5份呈现非特异性阳性反应。
