Oberhardt B J, Miller I F
Biophys J. 1972 Aug;12(8):933-47. doi: 10.1016/S0006-3495(72)86135-6.
The Polybrene method for detection of red cell antibodies which utilizes continuous flow equipment was modified so that kinetic studies could be performed on red cell antibodies doubly bound between adjacent red cells. In the anti-Rh(o)-Rh(o) erythrocyte system, deaggregation by temperature was studied over an antibody concentration range of from approximately 1 to 500 antibody molecules per erythrocyte, a residence time range of approximately eightfold, and a temperature range of from 10 to 55 degrees C. The rate of dissociation of antigen-antibody complex, as determined from deaggregation of antibody-dependent red cell aggregates, was found to be of apparent zero order. The apparent activation energy for the antigen-antibody reaction under the experimental conditions was determined and found to be higher than would be expected for singly bound antigen-antibody systems. Possible explanations are considered for these findings in terms of an antigen-antibody bond-breaking model.
利用连续流动设备检测红细胞抗体的聚凝胺方法进行了改进，以便能够对相邻红细胞之间双重结合的红细胞抗体进行动力学研究。在抗 - Rh（o）-Rh（o）红细胞系统中，研究了在每个红细胞约1至500个抗体分子的抗体浓度范围、约八倍的停留时间范围以及10至55摄氏度的温度范围内，温度对解聚的影响。从抗体依赖性红细胞聚集体的解聚情况确定的抗原 - 抗体复合物解离速率，被发现为表观零级。测定了实验条件下抗原 - 抗体反应的表观活化能，发现其高于单结合抗原 - 抗体系统的预期值。根据抗原 - 抗体断键模型，对这些发现的可能解释进行了探讨。
