Goldenberg D M, Pegram C A, Vazquez J J
J Immunol. 1975 Mar;114(3):1008-13.
An antigen has been isolated from a human signet-ring cell carcinoma serially growing in hamsters, GW-39, by saline, PCA, or phenol extraction, and has been found immunologically identical to a similarly extracted substance in normal human or hamster colon. No other hamster or human tissues or cells were found to contain this antigen, for which reason we have termed it colon-specific antigen, or CSA. CSA has been found to be distinct from the major blood group-specific antigens and from othercolon tumor-associated antigens, such as CEA, CCA-II, and CCA-III. It thus seems that a colon organ-specific antigen can be synthesized by this particular human tumor system. Hamsters immunized with CSA could reject cheek pouch grafts of GW-39 tumors, and tumor rejection by these animals correlated with their anti-CSA antibody titers. Preliminary characterization of CSA suggested that it is a glycoprotein on the cell surface having a molecular size of 30,000 to 50,000 daltons. It is proposed that CSA may play a role in the diagnosis of mucin-producing adenocarcinoma of the colon and in ulcerative colitis.
已通过盐水、PCA 或苯酚提取法从在仓鼠体内连续生长的人印戒细胞癌 GW-39 中分离出一种抗原，并且发现该抗原在免疫上与正常人和仓鼠结肠中类似提取的物质相同。未发现其他仓鼠或人体组织或细胞含有这种抗原，因此我们将其命名为结肠特异性抗原（CSA）。已发现 CSA 与主要血型特异性抗原以及其他结肠肿瘤相关抗原（如 CEA、CCA-II 和 CCA-III）不同。因此，似乎这种特定的人类肿瘤系统可以合成一种结肠器官特异性抗原。用 CSA 免疫的仓鼠可以排斥 GW-39 肿瘤的颊囊移植，并且这些动物的肿瘤排斥与其抗 CSA 抗体滴度相关。CSA 的初步特征表明它是一种细胞表面糖蛋白，分子大小为 30,000 至 50,000 道尔顿。有人提出，CSA 可能在结肠黏液腺癌和溃疡性结肠炎的诊断中发挥作用。
