Palumbo S A
J Bacteriol. 1972 Aug;111(2):430-6. doi: 10.1128/jb.111.2.430-436.1972.
Media and an analytical scheme have been developed which allow both a qualitative and quantitative estimation of the formation of pyocyanine, related phenazines, pyorubrin, and a blue and a yellow-green fluorescent pigment by Pseudomonas aeruginosa. Use of the defined pyocyanine medium of Frank and DeMoss with sulfate or various organic sulfur sources allowed formation of pyocyanine, related phenazines, and pyorubrin. When sulfite was the sulfur source with or without iron, P. aeruginosa formed either a yellow-green or a blue fluorescent pigment. Formation of fluorescent pigments of P. aeruginosa is related to the ability of sulfite to act as a specific sulfur source. In an investigation of the role of both added iron and sulfur sources, complex patterns of pigment formation were observed. In addition to the fluorescent pigments, sulfite also supported the formation of slime by P. aeruginosa.
已经开发出了培养基和一种分析方案,可对铜绿假单胞菌形成的绿脓菌素、相关吩嗪、脓红素以及一种蓝色和一种黄绿色荧光色素进行定性和定量评估。使用弗兰克和德莫斯定义的含硫酸盐或各种有机硫源的绿脓菌素培养基,可形成绿脓菌素、相关吩嗪和脓红素。当亚硫酸盐作为硫源且有或没有铁时,铜绿假单胞菌会形成黄绿色或蓝色荧光色素。铜绿假单胞菌荧光色素的形成与亚硫酸盐作为特定硫源的能力有关。在对添加的铁和硫源的作用进行的研究中,观察到了色素形成的复杂模式。除了荧光色素外,亚硫酸盐还支持铜绿假单胞菌形成黏液。