Al-Ani F Y, Al-Shibib A S, Khammas K M, Taher R
Folia Microbiol (Praha). 1986;31(3):215-9. doi: 10.1007/BF02928003.
Pure pyocyanin was prepared from 120 strains of Pseudomonas aeruginosa isolated from different clinical specimens. The modified method used increased the purification 425-fold. The spectra of pyocyanin of all strains were found to be identical with a single major peak at 363 nm. The only difference between these spectra appeared to be the level value absorbance. Strains recovered from different clinical specimens differ in their ability for pigment production. The strains isolated from urine produced higher amounts of pyocyanin than other isolates.
从120株从不同临床标本中分离出的铜绿假单胞菌中制备了纯绿脓菌素。所采用的改良方法使纯化倍数提高了425倍。发现所有菌株的绿脓菌素光谱相同，在363nm处有一个单一的主峰。这些光谱之间的唯一差异似乎是吸光度的水平值。从不同临床标本中分离出的菌株在色素产生能力上有所不同。从尿液中分离出的菌株产生的绿脓菌素比其他分离株更多。
