巨大芽孢杆菌中孢子形成的最低要求。
Minimal requirements for commitment to sporulation in Bacillus megaterium.
作者信息
Greene R A, Slepecky R A
出版信息
J Bacteriol. 1972 Aug;111(2):557-65. doi: 10.1128/jb.111.2.557-565.1972.
Abstract
Commitment to sporulation was examined by means of both endotrophic sporulation and rejuvenation experiments. In both cases, a point of commitment to the completion of sporulation occurred at stage II. With 2 x 10(8) cells/ml, the process by which commitment occurred required 0.5 mm Mg(2+), 10.0 mm phosphate, and an energy source (minimum of 5.0 mm acetate). For completion of sporulation and formation of normal, heat-resistant refractile spores, 0.3 mm Ca(2+) was required. Except for this Ca(2+) requirement, the completion of sporulation after commitment was independent of the nature of the surrounding medium. Some aspects of the utilization of these required substances were examined.
摘要
通过内营养型孢子形成和复壮实验来研究对孢子形成的 commitment。在这两种情况下，对孢子形成完成的 commitment 点出现在 II 阶段。对于每毫升 2×10⁸ 个细胞，发生 commitment 的过程需要 0.5 毫米的 Mg²⁺、10.0 毫米的磷酸盐和一种能量来源（至少 5.0 毫米的乙酸盐）。为了完成孢子形成并形成正常的、耐热的折光性孢子，需要 0.3 毫米的 Ca²⁺。除了这种对 Ca²⁺ 的需求外，commitment 后孢子形成的完成与周围培养基的性质无关。对这些所需物质的利用的一些方面进行了研究。
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