孢子库谷氨酸作为萌发过程中的一种代谢产物。
Spore pool glutamic acid as a metabolite in germination.
作者信息
Foerster H F
出版信息
J Bacteriol. 1972 Aug;111(2):437-42. doi: 10.1128/jb.111.2.437-442.1972.
Abstract
Spore glutamic acid pools were examined in dormant and germinating spores using colorimetric and (14)C analytical procedures. Germination of spores of Bacillus megaterium (parent strain), initiated by d-glucose, was accompanied by a rapid drop in the level of spore pool glutamate, from 12.0 mug/mg of dry spores to 7.7 mug/mg of dry spores after 30 sec of germination. Similar decreases in extractable spore pool glutamate were observed with l-alanine-initiated germination of B. licheniformis spores. On the other hand, glutamate pools of mutant spores of B. megaterium, with a requirement of gamma-aminobutyric acid for spore germination, remained unchanged for 9 min of germination, at which time more than 50% of the spore population had germinated. Evidence for conversion of spore pool glutamate to gamma-aminobutyric acid during germination of spores of B. megaterium (parent strain) was obtained.
摘要
利用比色法和（14）C分析方法，对休眠和萌发孢子中的孢子谷氨酸池进行了检测。巨大芽孢杆菌（亲本菌株）的孢子在d - 葡萄糖引发下萌发，伴随着孢子池谷氨酸水平的迅速下降，萌发30秒后，孢子池谷氨酸水平从12.0微克/毫克干孢子降至7.7微克/毫克干孢子。在地衣芽孢杆菌孢子的l - 丙氨酸引发的萌发过程中，也观察到可提取的孢子池谷氨酸有类似的下降。另一方面，巨大芽孢杆菌突变孢子的谷氨酸池，其孢子萌发需要γ - 氨基丁酸，在萌发9分钟内保持不变，此时超过50%的孢子群体已经萌发。获得了证据表明在巨大芽孢杆菌（亲本菌株）孢子萌发过程中孢子池谷氨酸转化为γ - 氨基丁酸。
相似文献
1
Spore pool glutamic acid as a metabolite in germination.孢子库谷氨酸作为萌发过程中的一种代谢产物。
J Bacteriol. 1972 Aug;111(2):437-42. doi: 10.1128/jb.111.2.437-442.1972.
2
-aminobutyric acid as a required germinant for mutant spores of Bacillus megaterium.γ-氨基丁酸作为巨大芽孢杆菌突变体孢子的必需萌发剂。
J Bacteriol. 1971 Nov;108(2):817-23. doi: 10.1128/jb.108.2.817-823.1971.
3
Glutamic acid decarboxylase in spores of Bacillus megaterium and its possible involvement in spore germination.巨大芽孢杆菌孢子中的谷氨酸脱羧酶及其在孢子萌发中的可能作用。
J Bacteriol. 1973 Jun;114(3):1090-8. doi: 10.1128/jb.114.3.1090-1098.1973.
4
Production of large amounts of acetate during germination of Bacillus megaterium spores in the absence of exogenous carbon sources.在没有外源碳源的情况下，巨大芽孢杆菌孢子萌发过程中产生大量乙酸盐。
J Bacteriol. 1977 Nov;132(2):744-6. doi: 10.1128/jb.132.2.744-746.1977.
5
EFFECT OF SPORULATION MEDIUM ON HEAT RESISTANCE, CHEMICAL COMPOSITION, AND GERMINATION OF BACILLUS MEGATERIUM SPORES.芽孢形成培养基对巨大芽孢杆菌芽孢耐热性、化学成分及萌发的影响
J Bacteriol. 1964 Apr;87(4):876-86. doi: 10.1128/jb.87.4.876-886.1964.
6
Physiological studies of an oligosporogenous strain of Bacillus popilliae.日本金龟子芽孢杆菌少孢菌株的生理学研究。
Appl Microbiol. 1971 Dec;22(6):1076-84. doi: 10.1128/am.22.6.1076-1084.1971.
7
Effects of glutamic acid on sporulation of Bacillus cereus and on spore properties.谷氨酸对蜡样芽孢杆菌芽孢形成及芽孢特性的影响。
Can J Microbiol. 1971 Apr;17(4):511-9. doi: 10.1139/m71-085.
8
Effects of alpha- and beta-D-glucose on germination of spores of Bacillus megaterium QM B1551.α-和β-D-葡萄糖对巨大芽孢杆菌QM B1551孢子萌发的影响。
J Biochem. 1979 Jan;85(1):315-8. doi: 10.1093/oxfordjournals.jbchem.a132327.
9
Properties of Bacillus megaterium and Bacillus subtilis mutants which lack the protease that degrades small, acid-soluble proteins during spore germination.巨大芽孢杆菌和枯草芽孢杆菌突变体的特性，这些突变体缺乏在孢子萌发过程中降解小的酸溶性蛋白质的蛋白酶。
J Bacteriol. 1992 Feb;174(3):807-14. doi: 10.1128/jb.174.3.807-814.1992.
10
Correlation between spore structure and spore properties in Bacillus megaterium.巨大芽孢杆菌中孢子结构与孢子特性之间的相关性
J Bacteriol. 1968 Jun;95(6):1979-86. doi: 10.1128/jb.95.6.1979-1986.1968.
引用本文的文献
1
Cloning of the gene for glutamate decarboxylase and its expression during conidiation in Neurospora crassa.谷氨酸脱羧酶基因的克隆及其在粗糙脉孢菌分生孢子形成过程中的表达。
Biochem J. 1993 Aug 1;293 ( Pt 3)(Pt 3):735-8. doi: 10.1042/bj2930735.
2
Regulation and glutamic acid decarboxylase during Neurospora crassa conidial germination.粗糙脉孢菌分生孢子萌发过程中的调控与谷氨酸脱羧酶
J Bacteriol. 1980 Dec;144(3):983-90. doi: 10.1128/jb.144.3.983-990.1980.
3
Regulation of aconitase synthesis in Bacillus subtilis: induction, feedback repression, and catabolite repression.枯草芽孢杆菌中乌头酸酶合成的调控：诱导、反馈阻遏和分解代谢物阻遏。
J Bacteriol. 1974 Mar;117(3):1295-305. doi: 10.1128/jb.117.3.1295-1305.1974.
4
Glutamic acid decarboxylase in spores of Bacillus megaterium and its possible involvement in spore germination.巨大芽孢杆菌孢子中的谷氨酸脱羧酶及其在孢子萌发中的可能作用。
J Bacteriol. 1973 Jun;114(3):1090-8. doi: 10.1128/jb.114.3.1090-1098.1973.
5
Repression of sporulation in Bacillus subtilis by L-malate.L-苹果酸对枯草芽孢杆菌孢子形成的抑制作用。
J Bacteriol. 1976 Feb;125(2):453-60. doi: 10.1128/jb.125.2.453-460.1976.
6
Spores of microorganisms. XXVI. Synthetic activities of germinating spores of Bacillus cereus prevented from outgrowth.微生物孢子。二十六。阻止生长的蜡样芽孢杆菌萌发孢子的合成活性。
Folia Microbiol (Praha). 1975;20(3):195-205. doi: 10.1007/BF02876779.
7
Neurospora crassa conidial germination: role of endogenous amino acid pools.粗糙脉孢菌分生孢子萌发：内源性氨基酸库的作用。
J Bacteriol. 1975 Oct;124(1):232-42. doi: 10.1128/jb.124.1.232-242.1975.
8
Metabolism and the triggering of germination of Bacillus megaterium. Concentrations of amino acids, organic acids, adenine nucleotides and nicotinamide nucleotides during germination.巨大芽孢杆菌的代谢与萌发触发。萌发过程中氨基酸、有机酸、腺嘌呤核苷酸和烟酰胺核苷酸的浓度。
Biochem J. 1978 Aug 15;174(2):627-34. doi: 10.1042/bj1740627.
9
Biochemical genetics of Neurospora crassa conidial germination.粗糙脉孢菌分生孢子萌发的生化遗传学
Bacteriol Rev. 1976 Mar;40(1):1-41. doi: 10.1128/br.40.1.1-41.1976.
本文引用的文献
1
Chromatography of amino acids on sulfonated polystyrene resins.氨基酸在磺化聚苯乙烯树脂上的色谱分析。
J Biol Chem. 1951 Oct;192(2):663-81.
2
Intermediate metabolism of aerobic spores. IV. Alanine deamination during the germination of spores of Bacillus cereus.需氧芽孢的中间代谢。IV. 蜡样芽孢杆菌芽孢萌发过程中的丙氨酸脱氨基作用。
J Bacteriol. 1959 Dec;78(6):844-51. doi: 10.1128/jb.78.6.844-851.1959.
3
A modified ninhydrin colorimetric analysis for amino acids.一种用于氨基酸的改良茚三酮比色分析法。
Arch Biochem Biophys. 1957 Mar;67(1):10-5. doi: 10.1016/0003-9861(57)90241-2.
4
Correlation of respiratory activity with phases of spore germination and growth in Bacillus megaterium as influenced by manganese and L-alanine.受锰和L-丙氨酸影响的巨大芽孢杆菌呼吸活性与孢子萌发及生长阶段的相关性
J Bacteriol. 1956 Aug;72(2):176-83. doi: 10.1128/jb.72.2.176-183.1956.
5
Analysis of respiration during germination and enlargement of spores of Bacillus megaterium and of the fungus Myrothecium verrucaria.巨大芽孢杆菌和疣孢漆斑菌孢子萌发及膨大过程中的呼吸分析。
J Gen Physiol. 1956 Jan 20;39(3):301-9. doi: 10.1085/jgp.39.3.301.
6
The stimulation of germination and respiration of Bacillus megaterium spores by mangasese, L-alanine and heat.锰、L-丙氨酸和热对巨大芽孢杆菌孢子萌发和呼吸的刺激作用。
J Bacteriol. 1955 Oct;70(4):368-74. doi: 10.1128/jb.70.4.368-374.1955.
7
Biochemical changes occurring during the germination of bacterial spores.细菌芽孢萌发过程中发生的生化变化。
Biochem J. 1953 May;54(2):205-9. doi: 10.1042/bj0540205.
8
-aminobutyric acid as a required germinant for mutant spores of Bacillus megaterium.γ-氨基丁酸作为巨大芽孢杆菌突变体孢子的必需萌发剂。
J Bacteriol. 1971 Nov;108(2):817-23. doi: 10.1128/jb.108.2.817-823.1971.
9
Biochemical studies of bacterial sporulation and germination. 23. Nucleotide metabolism during spore germination.细菌芽孢形成与萌发的生化研究。23. 芽孢萌发过程中的核苷酸代谢。
J Biol Chem. 1970 Jul 25;245(14):3645-52.
10
Biochemical studies of bacterial sporulation and germination. 18. Free amino acids in spores.细菌孢子形成与萌发的生化研究。18. 孢子中的游离氨基酸。
J Biol Chem. 1970 Mar 10;245(5):1128-36.
Zotero 插件