Freese E B, Cooney P, Freese E
Proc Natl Acad Sci U S A. 1975 Oct;72(10):4037-41. doi: 10.1073/pnas.72.10.4037.
The developmental stage at which cells of Bacillus megaterium are committed to continue differentiation, i.e., sporulation, depends on both the previous growth medium and the new medium to which the cells are transferred for the commitment test. The latest "stage of no return," after which cells continue differentiation, no matter how rich in nutrients the medium, is reached as soon as the forespore is completely surrounded by a double membrane.
巨大芽孢杆菌细胞致力于继续分化（即形成芽孢）的发育阶段，既取决于先前的生长培养基，也取决于用于进行分化确定试验而将细胞转接至的新培养基。最晚的“不可逆转阶段”是一旦前芽孢完全被双层膜包围即达到该阶段，此后细胞便继续分化，无论培养基营养多么丰富。
