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用于副球孢子菌病血清学诊断的间接荧光抗体和定量琼脂凝胶免疫扩散试验。

Indirect fluorescent-antibody and quantitative agar-gel immunodiffusion tests for the serological diagnosis of paracoccidioidomycosis.

作者信息

Restrepo A, Moncada L H

出版信息

Appl Microbiol. 1972 Jul;24(1):132-7. doi: 10.1128/am.24.1.132-137.1972.

Abstract

The value of various serological tests in the diagnosis of paracoccidioidomycosis was studied. Quantitative agar-gel immunodiffusion and indirect immunofluorescent tests were performed, and the results were compared with those of complement fixation and qualitative agar-gel procedures. The quantitative immunodiffusion procedure was found to serve as the simplest and safest quantitative test that could be performed for evaluation purposes, whereas the indirect fluorescent-antibody test gave nonspecific reactions and, as such, proved unsuitable.

摘要

研究了各种血清学检测在副球孢子菌病诊断中的价值。进行了定量琼脂凝胶免疫扩散和间接免疫荧光检测,并将结果与补体结合试验和定性琼脂凝胶检测的结果进行了比较。发现定量免疫扩散法是可用于评估目的的最简单、最安全的定量检测方法,而间接荧光抗体检测出现非特异性反应,因此证明不适用。

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