Marchette N J, Halstead S B, Nash D R, Stenhouse A C
Appl Microbiol. 1972 Sep;24(3):328-33. doi: 10.1128/am.24.3.328-333.1972.
A tissue explant culture technique for the recovery of dengue virus from experimentally infected monkey tissue is described and compared with tissue culture assay of tissue triturates and co-cultivation of trypsinized cells in cell cultures. The most efficient technique was one in which minced tissue was explanted in co-culture with dengue virus-susceptible LLC-MK2 monkey kidney cells. This technique shows promise of being useful for detection of virus in autopsy material from fatal dengue hemorrhagic fever cases.
本文描述了一种从实验感染的猴组织中分离登革病毒的组织外植体培养技术,并将其与组织研磨物的组织培养测定以及胰蛋白酶处理的细胞在细胞培养中的共培养进行了比较。最有效的技术是将切碎的组织与登革病毒易感的LLC-MK2猴肾细胞共培养。该技术有望用于检测登革出血热死亡病例尸检材料中的病毒。