Burgos J, Ordóñez J A, Sala F
Appl Microbiol. 1972 Sep;24(3):497-8. doi: 10.1128/am.24.3.497-498.1972.
Heat resistance of Bacillus cereus and Bacillus licheniformis spores in quarter-strength Ringer solution decreases markedly after ultrasonic treatments which are unable to kill a significant proportion of the spore population. This effect does not seem to be caused by a loss of Ca(2+) or dipicolinic acid. The use of ultrasonics to eliminate vegetative cells or to break aggregates in Bacillus spore suspensions to be used subsequently in heat resistance experiments appears to be unadvisable.
在四分之一强度的林格氏溶液中,蜡样芽孢杆菌和地衣芽孢杆菌孢子经超声处理后,其耐热性显著降低,而这种超声处理并不能杀死相当比例的孢子群体。这种效应似乎不是由钙离子或吡啶二羧酸的损失引起的。在随后用于耐热性实验的芽孢杆菌孢子悬液中,使用超声波消除营养细胞或打破聚集体似乎并不可取。