Shibata H, Uchida M, Hayashi H, Tani I
Microbiol Immunol. 1979;23(5):339-47. doi: 10.1111/j.1348-0421.1979.tb00471.x.
Spores of Bacillus cereus T treated with trichloroacetic acid (6.1--61.2 mM) were compared with untreated spores, and as the concentration of the chemical increased, the following alterations in spore properties were found: (1) the extent of germination decreased irrespective of the germination medium used; (2) the spores became sensitive to sodium hydroxide (1 N) and hydrochloric acid (0.27 N), but not to lysozyme (200 micrograms/ml); (3) loss of dipicolinate increased on subsequent heating; and (4) the spores became more sensitive to heat. However, trichloroacetic acid-treated spores were still viable and there was no significant change in spore components. The mechanism of action of trichloroacetic acid is discussed.
将用三氯乙酸（6.1 - 61.2 mM）处理过的蜡样芽孢杆菌T的孢子与未处理的孢子进行比较，随着该化学物质浓度的增加，发现孢子特性有以下变化：（1）无论使用何种萌发培养基，萌发程度均降低；（2）孢子对氢氧化钠（1 N）和盐酸（0.27 N）变得敏感，但对溶菌酶（200微克/毫升）不敏感；（3）后续加热时吡啶二羧酸的损失增加；（4）孢子对热变得更敏感。然而，经三氯乙酸处理的孢子仍具活力，且孢子成分无显著变化。本文讨论了三氯乙酸的作用机制。
