Hart P D, Armstrong J A, Brown C A, Draper P
Infect Immun. 1972 May;5(5):803-7. doi: 10.1128/iai.5.5.803-807.1972.
Mycobacterium lepraemurium and M. microti (causal agent of vole tuberculosis) were isolated from tissues of experimentally infected mice and used to infect normal mouse peritoneal macrophage cultures. The cellular response to these bacteria up to 4 days after infection was studied quantitatively by electron microscopy. Prelabeling with ferritin was used to facilitate observation of fusion between secondary lysosomes in the cells and phagosomes containing the bacteria. All bacteria were intraphagosomal, and a high proportion of them was morphologically "intact." Nearly all phagosomes containing morphologically damaged (presumed nonviable) bacteria also contained ferritin, having fused with secondary lysosomes. Fusion of lysosomes had also occurred with most phagosomes containing intact M. lepraemurium but was infrequent with phagosomes containing intact M. microti. This tendency of multiplying mycobacteria of the tubercle type to avoid contact with lysosomal contents has already been reported for M. tuberculosis strain H37Rv. The different intracellular circumstances of the parasites may reflect different means of intracellular survival.
从实验感染小鼠的组织中分离出鼠麻风分枝杆菌和微小分枝杆菌（田鼠结核病的病原体），并用于感染正常小鼠腹腔巨噬细胞培养物。通过电子显微镜对感染后长达4天的细胞对这些细菌的反应进行了定量研究。使用铁蛋白预标记来促进观察细胞中次级溶酶体与含有细菌的吞噬体之间的融合。所有细菌都在吞噬体内，并且其中很大一部分在形态上是“完整的”。几乎所有含有形态受损（推测无活力）细菌的吞噬体也都含有与次级溶酶体融合的铁蛋白。溶酶体与大多数含有完整鼠麻风分枝杆菌的吞噬体也发生了融合，但与含有完整微小分枝杆菌的吞噬体的融合很少见。结核型繁殖分枝杆菌避免与溶酶体内容物接触的这种倾向已经在结核分枝杆菌H37Rv菌株中有所报道。寄生虫不同的细胞内情况可能反映了不同的细胞内存活方式。
