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利用猪肾细胞培养的克隆系进行腺病毒的初次分离和疫苗研究。

Use of a clonal line of porcine kidney cell cultures for primary isolation and vaccine studies with adenoviruses.

作者信息

Mogabgab W J, Pollock B

出版信息

Appl Microbiol. 1972 Nov;24(5):760-2. doi: 10.1128/am.24.5.760-762.1972.

DOI:10.1128/am.24.5.760-762.1972
PMID:4629702
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380659/
Abstract

The clonal line (Y15) of porcine kidney stable cells provided a recovery system for adenovirus T4 from specimens from adults with respiratory illnesses that was as sensitive as human embryo kidney cultures. Adenoviruses T7 from adults, and T1, 2, 3, and 5 from children could be readily isolated in porcine kidney cell cultures. The latter were useful for adenovirus vaccine studies in that infectivity titers of live virus vaccine and neutralization antibody responses after vaccination were equal to those obtained in human embryo kidney cultures.

摘要

猪肾稳定细胞系(Y15)为从患有呼吸道疾病的成人标本中分离腺病毒T4提供了一个复苏系统,其敏感性与人类胚胎肾培养物相同。来自成人的腺病毒T7以及来自儿童的腺病毒T1、T2、T3和T5都能在猪肾细胞培养物中轻易分离出来。后者在腺病毒疫苗研究中很有用,因为活病毒疫苗的感染性滴度以及接种后的中和抗体反应与在人类胚胎肾培养物中获得的结果相当。

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本文引用的文献

1
Clonal line of porcine kidney stable cells for assay of Japanese encephalitis virus.用于乙型脑炎病毒检测的猪肾稳定细胞克隆系。
J Bacteriol. 1964 May;87(5):1239-40. doi: 10.1128/jb.87.5.1239-1240.1964.