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微量组织培养试验，用于在极少量人血清中滴定低浓度白喉抗毒素。

Microtissue culture test for the titration of low concentrations of diphtheria antitoxin in minimal amounts of human sera.

作者信息

Quevillon M, Chagnon A

出版信息

Appl Microbiol. 1973 Jan;25(1):1-4. doi: 10.1128/am.25.1.1-4.1973.

DOI:10.1128/am.25.1.1-4.1973
PMID:4631435
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380724/
Abstract

A microtissue culture method for the assay of low concentrations of diphtheria antitoxin in human sera has been developed, using a monkey kidney cell (VERO) culture technique. Results obtained with sera from nonvaccinated children and with immune sera from children vaccinated with three and four injections of diphtheria pertussis tetanus vaccine were in agreement with antitoxin levels considered necessary to denote immunity to diphtheria. The use of microplates and organic buffer for culturing the animal cells improved the stability of the tissue culture system. The described method is sensitive, economical, and applicable for the titration of antitoxin in human sera particularly from infants and children from whom a minimum amount of serum is available.

摘要

已开发出一种微组织培养方法，用于检测人血清中低浓度的白喉抗毒素，该方法采用猴肾细胞（VERO）培养技术。未接种疫苗儿童的血清以及接种三剂和四剂白喉百日咳破伤风疫苗的儿童的免疫血清所获得的结果，与认为对白喉具有免疫力所需的抗毒素水平一致。使用微孔板和有机缓冲液培养动物细胞提高了组织培养系统的稳定性。所描述的方法灵敏、经济，适用于滴定人血清中的抗毒素，特别是来自血清量最少的婴幼儿和儿童的血清。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/77b207393f70/applmicro00053-0017-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/1fd75ff952e4/applmicro00053-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/7649f674af73/applmicro00053-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/c7038612284a/applmicro00053-0017-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/77b207393f70/applmicro00053-0017-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/1fd75ff952e4/applmicro00053-0017-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/7649f674af73/applmicro00053-0017-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/c7038612284a/applmicro00053-0017-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/bb27/380724/77b207393f70/applmicro00053-0017-d.jpg

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Appl Microbiol. 1973 Jan;25(1):1-4. doi: 10.1128/am.25.1.1-4.1973.
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本文引用的文献

1
Titration of diphtheria toxin, antitoxin and toxoid in tissue culture.组织培养中白喉毒素、抗毒素和类毒素的滴定
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The action of diphtheria toxin on tissue cultures and its neutralization by antitoxin.白喉毒素对组织培养的作用及其被抗毒素中和的情况。
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Action of diphtheria toxin on cells cultivated in vitro.白喉毒素对体外培养细胞的作用。
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A micro tissue culture test for the titration and neutralization of rubella virus.
Can J Microbiol. 1969 Jan;15(1):67-71. doi: 10.1139/m69-011.
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Evaluation of 4-(2-hydroxyethyl)-1-piperazineëthanesulfonic acid (HEPES) as a tissue culture buffer.评估4-（2-羟乙基）-1-哌嗪乙磺酸（HEPES）作为组织培养缓冲液的性能。
Proc Soc Exp Biol Med. 1969 Jan;130(1):305-10. doi: 10.3181/00379727-130-33543.
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Use of a new buffer in the culture of animal cells.一种新缓冲液在动物细胞培养中的应用。
J Gen Virol. 1968 Mar;2(2):309-12. doi: 10.1099/0022-1317-2-2-309.
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Tissue culture titration of low concentrations of diphtheria antitoxin.低浓度白喉抗毒素的组织培养滴定法
Can J Public Health. 1971 Jul-Aug;62(4):345-7.
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Tissue culture method for the titration of diphtheria antitoxin in human sera.人体血清中白喉抗毒素滴定的组织培养方法。
Health Lab Sci. 1967 Jul;4(3):181-8.
10
Isolation and identification of adenoviruses in microplates.微孔板中腺病毒的分离与鉴定
Appl Microbiol. 1971 Nov;22(5):802-4. doi: 10.1128/am.22.5.802-804.1971.