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本文引用的文献

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Nutrition of animal cells in tissue culture; initial studies on a synthetic medium.组织培养中动物细胞的营养;对合成培养基的初步研究。
Proc Soc Exp Biol Med. 1950 Jan;73(1):1-8. doi: 10.3181/00379727-73-17557.
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DIFFERENTIATION IN VITRO OF EMBRYONIC CARTILAGE AND BONE IN A CHEMICALLY-DEFINED MEDIUM.在化学成分明确的培养基中对胚胎软骨和骨进行体外分化
Proc Soc Exp Biol Med. 1965 May;119:287-90. doi: 10.3181/00379727-119-30160.
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CLONAL GROWTH OF MAMMALIAN CELLS IN A CHEMICALLY DEFINED, SYNTHETIC MEDIUM.哺乳动物细胞在化学成分明确的合成培养基中的克隆生长
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CHEMICALLY DEFINED MEDIA FOR CULTIVATION OF LONG-TERM CELL STRAINS FROM FOUR MAMMALIAN SPECIES.用于培养来自四种哺乳动物的长期细胞株的化学成分确定培养基。
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SERUM ALBUMIN SUPPLEMENTED MEDIUM FOR LONG TERM CULTIVATION OF MAMMALIAN FIBROBLAST STRAINS.用于哺乳动物成纤维细胞系长期培养的血清白蛋白补充培养基。
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An improved nutrient solution for diploid Chinese hamster and human cell lines.一种改良的用于二倍体中国仓鼠和人类细胞系的营养液。
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The amino acid and protein metabolism of tissues cultivated in vitro.体外培养组织的氨基酸和蛋白质代谢
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Amino acid metabolism in mammalian cell cultures.哺乳动物细胞培养中的氨基酸代谢
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A protein growth factor for mammalian cells in culture.一种用于培养哺乳动物细胞的蛋白质生长因子。
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Monolayer cultures of trypsinized monkey kidney cells in synthetic medium; application to poliovirus synthesis.胰蛋白酶处理的猴肾细胞在合成培养基中的单层培养;在脊髓灰质炎病毒合成中的应用。
Proc Soc Exp Biol Med. 1956 Mar;91(3):464-70. doi: 10.3181/00379727-91-22294.

动物组织细胞在无血清的原代培养中的存活情况。

Survival of animal tissue cells in primary culture in the absence of serum.

作者信息

Orr H C, Baker J, Cheesman J O

出版信息

Appl Microbiol. 1973 Jan;25(1):49-54. doi: 10.1128/am.25.1.49-54.1973.

DOI:10.1128/am.25.1.49-54.1973
PMID:4631437
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC380734/
Abstract

The ability of cells from tissues of several species of animals to survive in primary culture without serum was tested. Of the species tested, cells from the kidneys of Macaca mulatta (rhesus) and Cercopithecus aethiops (vervet) monkeys and chicken embryo cells not only survived under these conditions, but indeed developed into confluent monolayer cultures. The addition of either serum or its globulin or albumin fraction enhanced the development of cell monolayers and permitted those cells unable to survive in the absence of serum to do so. Certain specific protein trypsin-inhibitors not of serum origin were unable to provide conditions necessary for cell survival or growth when used in place of serum proteins.

摘要

测试了几种动物组织的细胞在无血清的原代培养中存活的能力。在所测试的物种中,恒河猴和绿猴肾脏的细胞以及鸡胚细胞不仅在这些条件下存活,而且确实形成了汇合的单层培养物。添加血清、其球蛋白或白蛋白部分可促进细胞单层的形成,并使那些在无血清条件下无法存活的细胞能够存活。某些非血清来源的特定蛋白质胰蛋白酶抑制剂在代替血清蛋白使用时,无法提供细胞存活或生长所需的条件。