Norrby K, Franzén L
In Vitro. 1980 Jan;16(1):31-7. doi: 10.1007/BF02618197.
A procedure for the cultivation of mesentery is described, in which the culture is fully representative of the tissue of origin. The intact mesenteric membrane -- exposed to a minimum of trauma -- was spread out over a hole in a filter paper strip in fluid medium and was cultivated free-hanging. Specimens from rats and guinea pigs were used. The organ culture model appears especially apt for cytochemical and proliferation studies. Proliferation variables based on Feulgen DNA analysis in individual, morphologically defined cells and on mitotic counting and radiochemical analysis were estimated. The tissue was fully viable in chemically defined growth medium and showed an almost unaltered light microscopical appearance after up to 52 hr in culture.
本文描述了一种肠系膜培养方法，该培养物能充分代表其起源组织。将完整的肠系膜膜（尽量减少创伤）铺展在液体培养基中滤纸条的孔上，进行悬垂培养。使用了大鼠和豚鼠的样本。该器官培养模型似乎特别适合细胞化学和增殖研究。基于对单个形态学定义细胞的福尔根DNA分析、有丝分裂计数和放射化学分析，对增殖变量进行了评估。该组织在化学成分明确的生长培养基中完全存活，培养长达52小时后，光镜下外观几乎未改变。
