用于研究芽孢衣合成与形态发生的定量免疫测定法的开发。
Development of a quantitative immunological assay for the study of spore coat synthesis and morphogenesis.
作者信息
Horn D, Aronson A I, Golub E S
出版信息
J Bacteriol. 1973 Jan;113(1):313-21. doi: 10.1128/jb.113.1.313-321.1973.
Abstract
A quantitative assay employing (125)I-labeled antibody has been developed for Bacillus cereus T spore coat protein. Populations of antibody molecules with various affinities for inner or outer coat can be prepared by selective adsorption to and elution from different coat preparations. Adsorption to and elution from intact spores results in an antibody preparation at least 15 times more reactive to outer coat. This antibody is useful for measuring the time and extent of spore coat maturation, i.e., outer spore coat formation. Rifampin inhibits the increase in content of this coat antigen.
摘要
已开发出一种使用（125）I标记抗体的定量测定法来检测蜡样芽孢杆菌T芽孢衣壳蛋白。通过对不同衣壳制剂进行选择性吸附和洗脱，可以制备出对内衣壳或外衣壳具有不同亲和力的抗体分子群体。对完整孢子进行吸附和洗脱后得到的抗体制剂对外衣壳的反应性至少高15倍。这种抗体可用于测量芽孢衣壳成熟的时间和程度，即外芽孢衣壳的形成。利福平会抑制这种衣壳抗原含量的增加。
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
RNA AND PROTEIN SYNTHESIS REQUIRED FOR BACTERIAL SPORE FORMATION.细菌芽孢形成需要RNA和蛋白质合成。
Biochim Biophys Acta. 1964 Jun 22;87:267-76. doi: 10.1016/0926-6550(64)90222-1.
3
The composition and structure of bacterial spores.细菌芽孢的组成与结构。
J Cell Biol. 1963 Mar;16(3):579-92. doi: 10.1083/jcb.16.3.579.
4
Biochemical changes occurring during sporulation of Bacillus cereus. Inhibition of sporulation by alpha-picolinic acid.蜡样芽孢杆菌芽孢形成过程中发生的生化变化。α-吡啶甲酸对芽孢形成的抑制作用。
J Bacteriol. 1960 Jan;79(1):1-8. doi: 10.1128/jb.79.1.1-8.1960.
5
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J Infect Dis. 1958 Nov-Dec;103(3):239-62. doi: 10.1093/infdis/103.3.239.
6
Induction of immunological unresponsiveness to Vi antigen.
Ann Inst Pasteur (Paris). 1966 Jan;110(1):60-8.
7
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J Biol Chem. 1971 May 25;246(10):3189-95.
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