Stulting R D, Berke G
J Exp Med. 1973 Apr 1;137(4):932-42. doi: 10.1084/jem.137.4.932.
The binding of sensitized lymphocytes to tumor cells that leads to tumor cell lysis in vitro has been investigated using poly-L-lysine-fixed tumor cell monolayers and lymphocytes obtained from the anatomical site of tumor allograft rejection. The results show that magnesium is an important prerequisite for this interaction and that the extent of lymphocyte-tumor cell binding depends upon temperature as well as pH. Binding can occur in the absence of serum, although serum factors are necessary for the completion of the cytolytic process. The poly-L-lysine technique is applicable to the formation of confluent monolayers with virtually any normal or neoplastic cell type, including those that are otherwise nonadherent to surfaces. Cells immobilized by this technique can be used for the specific immunoabsorption and subsequent recovery of effector lymphocytes sensitized against transplantation or tumor cell antigens.
利用聚-L-赖氨酸固定的肿瘤细胞单层以及从肿瘤同种异体移植排斥反应解剖部位获取的淋巴细胞，研究了致敏淋巴细胞与肿瘤细胞的结合，这种结合在体外会导致肿瘤细胞裂解。结果表明，镁是这种相互作用的重要前提条件，淋巴细胞与肿瘤细胞的结合程度取决于温度以及pH值。尽管血清因子对于细胞溶解过程的完成是必需的，但在无血清的情况下也能发生结合。聚-L-赖氨酸技术适用于与几乎任何正常或肿瘤细胞类型形成汇合单层，包括那些原本不粘附于表面的细胞。通过该技术固定的细胞可用于特异性免疫吸附以及随后回收针对移植或肿瘤细胞抗原致敏的效应淋巴细胞。
