Pösö A R, Pösö H
Acta Chem Scand B. 1979;33(4):249-55. doi: 10.3891/acta.chem.scand.33b-0249.
The rate of ethanol elimination in vivo was studied with rats in which the energy consumption of the liver was increased by partial hepatectomy. Immediately after partial hepatectomy the activity of alcohol dehydrogenase in the liver remnant was not changed from that of the livers of sham-operated controls, but the rate of ethanol removal was significantly faster. Twenty-four h after the partial hepatectomy the activity of alcohol dehydrogenase was only 48 % of the activity measured in unoperated control rats. Therefore it is concluded that in normal liver the activity of ADH is in excess. In partially hepatectomized rats the rate of ethanol elimination was linearly correlated with the activity of alcohol dehydrogenase, which suggests that when the rate of NADH reoxidation is markedly increased, as in regenerating rat liver, the rate of ethanol elimination may be limited by the activity of alcohol dehydrogenase. The activity of aldehyde dehydrogenase and the concentration of acetaldehyde in the tail blood were not significantly changed from the level of unoperated rats during oxidation of ethanol.
通过部分肝切除术增加肝脏能量消耗的大鼠，研究了体内乙醇的消除速率。部分肝切除术后立即观察到，肝残余组织中乙醇脱氢酶的活性与假手术对照组的肝脏相比没有变化，但乙醇清除率明显更快。部分肝切除术后24小时，乙醇脱氢酶的活性仅为未手术对照大鼠测得活性的48%。因此得出结论，在正常肝脏中，乙醇脱氢酶的活性是过量的。在部分肝切除的大鼠中，乙醇消除率与乙醇脱氢酶的活性呈线性相关，这表明当NADH再氧化速率显著增加时，如在再生的大鼠肝脏中，乙醇消除率可能受乙醇脱氢酶活性的限制。在乙醇氧化过程中，醛脱氢酶的活性和尾静脉血中乙醛的浓度与未手术大鼠相比没有显著变化。
