Polosatov M V, Klimov P K, Masevich C G, Samartsev M A, Wünsch E
Acta Hepatogastroenterol (Stuttg). 1979 Apr;26(2):154-9.
Radio gel and affin chromatography were used to study big gastrin interaction with blood proteins of man and animals. The experiments showed that big gastrin interacts with serum proteins in vitro. The gastrin-blood protein complex is labile and readily dissociates (T 1/2 = 8--14 min). A more stable complex is found in acidic medium. Ceruloplasmin is one of the blood serum proteins able to interact with big gastrin. The stability of hormone-protein complex, formed by gastrin and ceruloplasmin, is dependent upon hormone concentration. With the addition of C-terminal penta--and octapeptides to labelled hormone, binding increased. It is speculated that formation of labile gastrin-blood protein complexes is necessary for selective gastrin transport from hormone-producing cells to target cells.
采用放射凝胶和亲和色谱法研究了大胃泌素与人及动物血液蛋白质的相互作用。实验表明，大胃泌素在体外可与血清蛋白相互作用。胃泌素 - 血液蛋白复合物不稳定，易于解离（半衰期 = 8 - 14分钟）。在酸性介质中可发现更稳定的复合物。铜蓝蛋白是能够与大胃泌素相互作用的血清蛋白之一。由胃泌素和铜蓝蛋白形成的激素 - 蛋白质复合物的稳定性取决于激素浓度。向标记激素中添加C末端五肽和八肽后，结合增加。推测不稳定的胃泌素 - 血液蛋白复合物的形成对于胃泌素从产生激素的细胞向靶细胞的选择性转运是必要的。
