Properties and distribution of peripherally evoked presynaptic hyperpolarization in cat lumbar spinal cord.

1. The action of peripheral nerve volleys on the polarization of presynaptic terminals of inactive sensory fibres in cat lumbar spinal cord has been investigated by recording (a) the dorsal root potential (DRP), (b) intracellular changes in polarization of single preterminal axons (PAD or PAH), and (c) changes in excitability of populations of preterminal axons.2. Presynaptic hyperpolarization (positive DRP-PAH) can be evoked by stimulation of muscle group III afferents as well as by volleys in cutaneous Abeta, Adelta and C afferents. These volleys can also produce presynaptic depolarization (negative DRP-PAD).3. The positive DRP is observed in the decerebrate state and increases in amplitude following spinalization.4. Picrotoxin blocks the positive DRP at the same dosages required to block the negative DRP. Test negative DRPs are depressed during a conditioning positive DRP. These results are used to support earlier suggestions that the positive DRP results from inhibition of interneurones mediating the negative DRP.5. Trains of group III stimuli at 20/sec evoke a steady positive DRP. Trains of the same intensity at 200/sec evoke a phasic negative DRP. This frequency dependence is observed for PAD and PAH in single sensory axons.6. The DRPs recorded from different dorsal root filaments in response to a given stimulus vary widely in the ratio of negative to positive DRP.7. Intracellular recording from single axons reveals that the same stimuli evoke widely varying ratios of PAD and PAH.8. Stimulation of FRA evokes PAH > PAD in PBST group I afferents, PAD > PAH in sural A fibres and intermediate effects in G-S group I units.9. It is suggested that activation of flexor reflex afferents may selectively potentiate the synaptic efficacy of large muscle afferents mediating the flexor reflex rather than large skin afferents or large afferents from extensor muscles.