Brown T D, Pereira C R, Stormer F C
J Bacteriol. 1972 Dec;112(3):1106-11. doi: 10.1128/jb.112.3.1106-1111.1972.
Mutants of Aerobacter aerogenes devoid of acetate kinase and phosphotransacetylase activities were isolated by selection for resistance to fluoroacetate on lactate medium. The mutants were used to study the role of the acetate kinase-phosphotransacetylase system in growth on acetate and glucose. Acetate kinase-negative and phosphotransacetylase-negative mutants were unable to grow on acetate minimal medium. Their growth rates on glucose minimal medium were identical with that of the parent strain under aerobic conditions, but lower growth rates were observed in the mutant strains during anaerobic growth on glucose medium. The mutants were unable to incorporate [2-(14)C]-acetate rapidly while growing on glycerol. Variations in acetate kinase and phosphotransacetylase levels during growth on glucose were studied. The specific activities of the enzymes increased approximately fivefold during aerobic growth on glucose in batch culture. The enzyme levels were also studied during anaerobic growth on glucose at constant pH (pH 5.8 and 7.0). Smaller increases in specific activities were found under these conditions. The role of acetate in the induction of the diacetyl (acetoin) reductase was investigated using a mutant deficient in both acetate kinase and phosphotransacetylase. The effect of pH on the induction of this enzyme during growth on glucose under anaerobic conditions was tested. The data support the idea that free acetic acid is the inducer for the enzymes of the butanediol-forming pathway in A. aerogenes.
通过在乳酸培养基上筛选对氟乙酸的抗性，分离出了缺乏乙酸激酶和磷酸转乙酰酶活性的产气气杆菌突变体。这些突变体被用于研究乙酸激酶 - 磷酸转乙酰酶系统在乙酸和葡萄糖生长过程中的作用。乙酸激酶阴性和磷酸转乙酰酶阴性的突变体无法在乙酸基本培养基上生长。在需氧条件下，它们在葡萄糖基本培养基上的生长速率与亲本菌株相同，但在葡萄糖培养基上厌氧生长期间，突变菌株的生长速率较低。这些突变体在以甘油为碳源生长时无法快速掺入[2-(14)C] - 乙酸。研究了在葡萄糖上生长期间乙酸激酶和磷酸转乙酰酶水平的变化。在分批培养中，在葡萄糖上需氧生长期间，这些酶的比活性增加了约五倍。还研究了在恒定pH（pH 5.8和7.0）下在葡萄糖上厌氧生长期间的酶水平。在这些条件下，发现比活性的增加较小。使用同时缺乏乙酸激酶和磷酸转乙酰酶的突变体研究了乙酸在二乙酰（乙偶姻）还原酶诱导中的作用。测试了pH对厌氧条件下在葡萄糖上生长期间该酶诱导的影响。数据支持游离乙酸是产气气杆菌中丁二醇形成途径酶的诱导剂这一观点。
