采用多离子监测气相色谱-质谱联用技术对DNA中5-甲基胞嘧啶进行灵敏检测并定量测定5-甲基胞嘧啶与胞嘧啶的比例 - Suppr

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Sensitive detection of 5-methylcytosine and quantitation of the 5-methylcytosine/cytosine ratio in DNA by gas chromatography--mass spectrometry using multiple specific ion monitoring.

作者信息

Singer J, Schnute W C, Shively J E, Todd C W, Riggs A D

出版信息

Anal Biochem. 1979 Apr 15;94(2):297-301. doi: 10.1016/0003-2697(79)90363-4.

DOI:10.1016/0003-2697(79)90363-4

PMID:464298

Abstract

摘要

相似文献

Sensitive detection of 5-methylcytosine and quantitation of the 5-methylcytosine/cytosine ratio in DNA by gas chromatography--mass spectrometry using multiple specific ion monitoring.采用多离子监测气相色谱-质谱联用技术对DNA中5-甲基胞嘧啶进行灵敏检测并定量测定5-甲基胞嘧啶与胞嘧啶的比例

Anal Biochem. 1979 Apr 15;94(2):297-301. doi: 10.1016/0003-2697(79)90363-4.

Distribution of 5-methylcytosine in the DNA of somatic and germline cells from bovine tissues.牛组织中体细胞和生殖细胞DNA中5-甲基胞嘧啶的分布。

Nucleic Acids Res. 1981 Sep 25;9(18):4537-46. doi: 10.1093/nar/9.18.4537.

Analysis of modified bases in DNA by stable isotope dilution gas chromatography-mass spectrometry: 5-methylcytosine.

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Analysis of base composition of RNA and DNA hydrolysates by gas-liquid chromatography.通过气液色谱法分析RNA和DNA水解产物的碱基组成。

J Chromatogr. 1971 Nov 26;62(3):347-67. doi: 10.1016/s0021-9673(00)91386-4.

Absence of detectable 5-methylcytosine in DNA of embryos of the brine shrimp, Artemia.卤虫胚胎DNA中未检测到5-甲基胞嘧啶。

Dev Biol. 1984 Mar;102(1):264-7. doi: 10.1016/0012-1606(84)90191-x.

Analysis of 5-methylcytosine in DNA. I. Mass spectrometry.DNA中5-甲基胞嘧啶的分析。I. 质谱分析。

Anal Biochem. 1976 May 7;72:586-92. doi: 10.1016/0003-2697(76)90570-4.

Gas chromatography-mass spectrometry characteristics of methylated perfluoroacyl derivatives of cytosine and 5-methylcytosine.胞嘧啶和5-甲基胞嘧啶的甲基化全氟酰基衍生物的气相色谱-质谱特征

J Chromatogr. 1984 Nov 30;314:211-7. doi: 10.1016/s0021-9673(01)97735-0.

Trace detection of modified DNA bases via moving-belt liquid chromatography-mass spectrometry using electrophoric derivatization and negative chemical ionization.通过使用电泳衍生化和负化学电离的移动带液相色谱-质谱法对修饰的DNA碱基进行痕量检测。

J Chromatogr. 1989 Mar 31;465(2):285-96. doi: 10.1016/s0021-9673(01)92666-4.

Methylation of reiterated sequences in mammalian DNAs. Effects of the tissue type, age, malignancy and hormonal induction.哺乳动物DNA中重复序列的甲基化。组织类型、年龄、恶性肿瘤及激素诱导的影响。

Biochim Biophys Acta. 1981 Apr 27;653(2):204-18. doi: 10.1016/0005-2787(81)90156-8.

Bovine thymus satellite I DNA sequences, which are highly methylated, are preferentially located in H1-rich chromatin.高度甲基化的牛胸腺卫星I DNA序列优先位于富含H1的染色质中。

Biochem Cell Biol. 1988 Apr;66(4):256-61. doi: 10.1139/o88-034.

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Engineering a high-affinity methyl-CpG-binding protein.构建一种高亲和力的甲基化CpG结合蛋白。

Nucleic Acids Res. 2006 Aug 7;34(13):e96. doi: 10.1093/nar/gkl527.

Sequence arrangement of a highly methylated satellite DNA of a plant, Scilla: A tandemly repeated inverted repeat.植物菝葜高度甲基化卫星 DNA 的序列排列：串联重复的反向重复。

Proc Natl Acad Sci U S A. 1981 Jan;78(1):338-42. doi: 10.1073/pnas.78.1.338.

Identifying 5-methylcytosine and related modifications in DNA genomes.识别DNA基因组中的5-甲基胞嘧啶及相关修饰。

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Quantitative reversed-phase high performance liquid chromatographic determination of major and modified deoxyribonucleosides in DNA.反相高效液相色谱法定量测定DNA中的主要和修饰脱氧核糖核苷。

Nucleic Acids Res. 1980 Oct 24;8(20):4763-76. doi: 10.1093/nar/8.20.4763.

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