Mohamed G B, Nazareth A, Hayes M J, Giese R W, Vouros P
J Chromatogr. 1984 Nov 30;314:211-7. doi: 10.1016/s0021-9673(01)97735-0.
Pentafluorobenzoyl and heptafluorobutyryl derivatives of cytosine and pentafluorobenzoyl-5-methylcytosine have been examined by mass spectrometry following permethylation. Electron impact mass spectra were used to confirm the structures of the derivatives. The permethylated pentafluorobenzoyl derivatives of cytosine and 5-methylcytosine are sensitive for quantitative analysis by capillary gas chromatography-mass spectrometry. Under negative ion chemical ionization conditions 1 femtogram of each is detected.
胞嘧啶的五氟苯甲酰基和七氟丁酰基衍生物以及五氟苯甲酰基-5-甲基胞嘧啶在全甲基化后通过质谱法进行了研究。电子轰击质谱用于确认衍生物的结构。胞嘧啶和5-甲基胞嘧啶的全甲基化五氟苯甲酰基衍生物对毛细管气相色谱-质谱法定量分析敏感。在负离子化学电离条件下，每种物质可检测到1飞克。
