Schlumpf M, Lichtensteiger W
Anat Embryol (Berl). 1979 Jun 5;156(2):177-87. doi: 10.1007/BF00300013.
Catecholamines were found histochemically in the visceral yolk sac of the rat from embryonic day (ED) 10, i.e. before the amines become detectable in peripheral or central neurons of the fetus. Formaldehyde-induced fluorescence was confined to the apical part of the yolk sac epithelial cells. The specificity of histofluorescence has been confirmed by borohydride reduction, microspectrofluorimetry revealing an emission peak at 480 nm and administration of reserpine. The catecholamines present were identified by mass fragmentography using N,O-trifluoroacetyl derivatives. At ED 13 both dopamine and norepinephrine were present, while only dopamine was detected at ED 18 1/2. Maternal circulation or the epithelial cells themselves appear as possible sources of these catecholamines. The occurrence of amines in the yolk sac epithelium may reflect an intracellular role of these compounds, a barrier function of the epithelium or a step in a transport to the fetus where the amines might assume regulatory functions.
从胚胎第10天起，在大鼠的内脏卵黄囊中通过组织化学方法发现了儿茶酚胺，即在胎儿外周或中枢神经元中可检测到胺类之前。甲醛诱导的荧光局限于卵黄囊上皮细胞的顶端部分。硼氢化还原、显示480nm发射峰的显微分光荧光测定法以及利血平的给药已证实了组织荧光的特异性。使用N,O-三氟乙酰衍生物通过质量碎片分析法鉴定了存在的儿茶酚胺。在胚胎第13天，多巴胺和去甲肾上腺素都存在，而在胚胎第18.5天仅检测到多巴胺。母体循环或上皮细胞本身似乎是这些儿茶酚胺的可能来源。卵黄囊上皮中胺类的出现可能反映了这些化合物的细胞内作用、上皮的屏障功能或向胎儿转运的一个步骤，在胎儿中胺类可能发挥调节功能。
