Kuusi T, Schröder T, Bång B, Lempinen M, Ehnholm C
Biochim Biophys Acta. 1979 Jun 21;573(3):443-50. doi: 10.1016/0005-2760(79)90219-4.
Immunochemical methods for the selective measurement of pig post-heparin plasma lipoprotein lipase and hepatic lipase are described and validated. A simple two step purification method for porcine hepatic lipase from hepatic perfusate based on affinity chromatography and gel filtration is reported. The activity of the post-heparin plasma lipoprotein lipase and hepatic lipase in swine is reported. It is demonstrated that fasting decreases the activity of post-heparin plasma lipoprotein lipase activity more than two-fold while it does not affect the hepatic lipase activity significantly.
描述并验证了用于选择性测量猪肝素后血浆脂蛋白脂肪酶和肝脂肪酶的免疫化学方法。报道了一种基于亲和色谱和凝胶过滤从肝脏灌流液中简单两步纯化猪肝脏脂肪酶的方法。报道了猪肝素后血浆脂蛋白脂肪酶和肝脂肪酶的活性。结果表明，禁食使肝素后血浆脂蛋白脂肪酶活性降低两倍以上，而对肝脂肪酶活性无明显影响。
